Journal of Biotechnology & Biomaterials
Open Access

Abstract

Collagen is a protein rich tissue that is found in the bodies of animals (and of course humans). Collagen is abundant within the body, and makes up a total of 25% of protein readily available. Collagen is usually associated with cosmetic surgery, and as a burn healing agent. Collagen was prepared from the fish waste by extraction with cold 0.5M acetic acid and purification by alternative precipitation with sodium chloride and dialysis against 0.02M disodium hydrogen phosphate. The purified collagen was characterized with the help of SDS-PAGE. In this work, we describe a cost effective and environment friendly technique for the synthesis of silver nanoparticles from 1mM AgNo3 solution through the extract of collagen obtained from fish waste. Nanoparticles were characterized using UV-Vis absorption spectroscopy, FTIR. Further these biologically synthesized nanoparticles have found to be highly toxic against different multi drug resistant human pathogens. Bacteria such as Escherichia coli, Bacillus subtilis & Pseudomonas aeruginosa were used to test their antibiotic activity against the silver nanoparticle synthesized using fish collagen. Collagen has been reported as a characteristic suitable for medical application such as degrability, nontoxic, weak antigenicity and has been used in resorbable sutures, haemostatic agents. It can be processed into variety of forms and it is readily isolated and purified in large quantities. Our preliminary in vitro study report of fish collagen with silver nanoparticle is proved to be more effective against bacteria.

Biography

Ramya R has completed her M. Sc (Biotechnology) at the age of 22 years from Lady Doak College, Madurai and now she is doing her M.Phil (Biotechnology) in Lady Doak College, Madurai.