Edward Cedrone | OMICS International
ISSN: 2576-3881

Journal of Cytokine Biology
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Edward Cedrone


Chemical Modification of CRISPR gRNAs Eliminate type I Interferon Responses in Human Peripheral Blood Mononuclear Cells

Objectives: CRISPR/Cas9 is currently the primary tool used for genome editing in mammalian cells. To cleave and alter genomic DNA, both the Cas9 nuclease and a guide RNA (gRNA) must be present in the nucleus. One preferred method of introducing these reagents is direct transfection of a recombinant Cas9 protein complexed with a synthetic gRNA as a ... Read More»

Mollie S. Schubert, Edward Cedrone, Barry Neun, Mark A. Behlke and Marina A. Dobrovolskaia

Case Report: J Cytokine Biol, 3: 121

DOI: 10.4172/2576-3881.1000121

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