alexa Blood and Tissue Leukocyte Apoptosis in Trypanosoma brucei Infected Rats | OMICS International | Abstract
E-ISSN: 2314-7326
P-ISSN: 2314-7334

Journal of Neuroinfectious Diseases
Open Access

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Blood and Tissue Leukocyte Apoptosis in Trypanosoma brucei Infected Rats

Anise N. Happi1,* Danny A. Milner Jr.2 and Richard E. Antia1

1Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria

2Department of Pathology, Brigham and Women’s Hospital, Boston, MA 02115, USA

*Corresponding Author:
Anise N. Happi
Department of Veterinary Pathology
Faculty of Veterinary Medicine
University of Ibadan, Ibadan, Nigeria
E-mail: [email protected]

Received Date: 29 January 2012 Revised Date: Revised 11 April 2012 Accepted Date: 16 April 2012

Abstract

Severity of disease in human African trypanosomiasis may be linked, as in other human protozoan infections, to apoptosis of host inflammatory cells during infection. The present study investigates the involvement of leukocyte apoptosis in Trypanosoma brucei infection of rodents. Twenty-seven Wistar rats were infected with 1 × 103 T. brucei and leukocyte apoptosis was evaluated by several methods. Apoptotic cell count was performed in blood, spleen, thymus, lymph node and liver during infections, using Light Microscopy (LM), agarose gel electrophoresis and Transmission Electron Microscopy (TEM). Blood and tissues leukocyte apoptosis in infected animals were confirmed by LM, TEM, and agarose gel electrophoresis, which showed low molecular weight DNA fragments. Infected rats sacrificed after day 8 PI showed significant increase in apoptotic cells in both blood (p<0.001) and spleen (p < 0.05). Peak blood leukocyte apoptosis corresponded with peak parasitemia and the lowest total leukocyte count in all infected rats. Our data provide the first documentation of increased levels of blood leukocyte apoptosis during T. brucei infection. Apoptosis of blood cells during trypanosome infection may represent a putative mechanism for the severity of leukopenia and disease.

Keywords

Recommended Conferences

International Congress on Neurology

Auckland, New Zealand
Share This Page
Top