alexa Development of Three Real-Time PCR assays to Detect Bacillus anthracis and Assessment of Diagnostic Utility | OMICS International | Abstract
ISSN: 2157-2526

Journal of Bioterrorism & Biodefense
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Development of Three Real-Time PCR assays to Detect Bacillus anthracis and Assessment of Diagnostic Utility

Tanya M. Parsons, Victoria Cox, Angela Essex-Lopresti, Margaret G. Hartley, Roman A. Lukaszewski, Phillip A. Rachwal, Helen L. Stapleton and Simon A. Weller*

Defence Science and Technology Laboratory, Dstl Porton Down, Salisbury, UK

*Corresponding Author:
Simon A. Weller
Defence Science and Technology Laboratory
Dstl Porton Down, Salisbury, UK
Tel: +44 (0) 1980617404
E-mail: [email protected]

Received Date: December 01, 2012; Accepted Date: January 18, 2013; Published Date: January 23, 2013

Citation: Parsons TM, Cox V, Essex-Lopresti A, Hartley MG, Lukaszewski RA, et al. (2013) Development of Three Real-Time PCR assays to Detect Bacillus anthracis and Assessment of Diagnostic Utility. J Bioterr Biodef S3:009. doi: 10.4172/2157-2526.S3-009

Copyright: © 2013 Parsons TM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Three real-time PCR assays to detect Bacillus anthracis genetic targets (pXO1; pXO2 and chromosome) were developed. Two of the PCR assays (pXO1-MGB and Ba chr-MGB) were tested against DNA extracts produced from whole blood samples obtained from a replicated B. anthracis murine infection model. Across all three models 45 samples were tested in total, within which a subset of 41 samples were shown to contain B. anthracis by either PCR or microbiological culture. Using microbiological culture as an analogue of conventional blood culture (as used in clinical settings) the detection rates of PCR and blood culture were compared. In two of the murine models blood culture had a significantly higher detection rate than PCR (BA1, p=0.004; BA3, p=0.013). In the BA2 model there was no significant difference between the detection rates of PCR and blood culture.

Keywords

Recommended Conferences
Share This Page
Top