Development of Three Real-Time PCR assays to Detect Bacillus anthracis and Assessment of Diagnostic Utility
Tanya M. Parsons, Victoria Cox, Angela Essex-Lopresti, Margaret G. Hartley, Roman A. Lukaszewski, Phillip A. Rachwal, Helen L. Stapleton and Simon A. Weller*
Defence Science and Technology Laboratory, Dstl Porton Down, Salisbury, UK
- *Corresponding Author:
- Simon A. Weller
Defence Science and Technology Laboratory
Dstl Porton Down, Salisbury, UK
Tel: +44 (0) 1980617404
E-mail: [email protected]
Received Date: December 01, 2012; Accepted Date: January 18, 2013; Published Date: January 23, 2013
Citation: Parsons TM, Cox V, Essex-Lopresti A, Hartley MG, Lukaszewski RA, et al. (2013) Development of Three Real-Time PCR assays to Detect Bacillus anthracis and Assessment of Diagnostic Utility. J Bioterr Biodef S3:009. doi: 10.4172/2157-2526.S3-009
Copyright: © 2013 Parsons TM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Three real-time PCR assays to detect Bacillus anthracis genetic targets (pXO1; pXO2 and chromosome) were developed. Two of the PCR assays (pXO1-MGB and Ba chr-MGB) were tested against DNA extracts produced from whole blood samples obtained from a replicated B. anthracis murine infection model. Across all three models 45 samples were tested in total, within which a subset of 41 samples were shown to contain B. anthracis by either PCR or microbiological culture. Using microbiological culture as an analogue of conventional blood culture (as used in clinical settings) the detection rates of PCR and blood culture were compared. In two of the murine models blood culture had a significantly higher detection rate than PCR (BA1, p=0.004; BA3, p=0.013). In the BA2 model there was no significant difference between the detection rates of PCR and blood culture.