Integrins Contributes to Innate Immune Response in Pelteobagrus
Ming-Ming Han1,2*, Jian-Guo Lu1, Shang-Bin1, Li-Na-Peng1, Shahid Mahboob3,4, Khalid A Al-Ghanim3 and Xiao-Wen Sun1
1Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, China
2Shanghai Ocean University, College of Fisheries and Life Science, Shanghai, China
3Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
4Department of Zoology, GC University, Faisalabad, Pakistan
- *Corresponding Author:
- Ming-Ming Han
Heilongjiang River Fisheries Research Institute
Chinese Academy of Fishery Sciences, Harbin, China
E-mail: [email protected]
Received date: March 15, 2016; Accepted date: April 27, 2016; Published date: May 05, 2016
Citation: Han MM, Lu JG, Bin S, Peng LN, Mahboob S, et al. (2016) Integrins Contributes to Innate Immune Response in Pelteobagrus Fulvidraco. Biochem Physiol 5:204. doi:10.4172/2168-9652.1000204
Copyright: © 2016 Han MM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Integrins - β are highly conserved pleiotropic cytokine. In this study, we cloned the full - length cDNA sequence of yellow catfish Pelteobagrus fulvidraco integrin - β1 and integrin - β3 gene. The Integrin - β1 cDNA is 2816 bp in length and encodes a protein of 461 amino acids. Integrin - β3 cDNA is 1713 bp in length and encodes 479 amino acids. Integrins - β were classified strictly with the same type of proteins by phylogenetic analysis. Stegastes partitus integrins - β was classified on the same phylogenetic branch compared with other fish. Tissue transcription profile analysis indicated that, in the healthy P. fulvidraco, integrins - β mRNA was predominantly expressed in the liver and brain, follow by gill and spleen. The P. fulvidraco integrins - β shares high homology with the Cypriniformes Danio rerio (83% ~87%). Expression of the two Integrins - β transcripts showed significant expression changes in 4 h after infection with Flavobacterium columnare, which has modulation of expression continuing up to 3 d following pathogen exposure. Both of the integrin - β genes were significantly up - regulated at 24 h in spleen and liver after infection tissue. Integrin - β3 showed the most sensitive to F. columnare, suppressing expression - 6.34 - fold at 24h. This informations elucidates the functions of individual integrins - β genes in regards to pathogen recognition, binding, and their larger immune context.