Journal of Earth Science & Climatic Change
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Environmental pollution implies any alterations in the surroundings, but it is restricted in use especially to mean any
deterioration in the physical, chemical and biological quality of the environment. The contamination of the environment
with crude oil results in environmental pollution. Most of the physicochemical and thermal methods are expensive, as they
require expensive equipment and machineries and expend good amount of energy. Among these procedures, bioremediation
is currently associated to physicochemical procedures. Soil sample was collected from coastal area (Mumbai).
was isolated from petroleum contaminated soil using selective enrichment technique. Hydrocarbon substrate (4T engine oil)
was used as a carbon source (1% v/v). The GC/MS analysis was performed using a MS 5973 spectrometer coupled to 9 Hewlett
Packard model 6890. Among the isolated microorganisms this strain was identified by 16s rRNA sequencing and it was able to
degrade, 81% of 4T engine oil. At the end of the experiments, GC analysis showed significant differences in the composition
of hydrocarbons in engine oil.
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