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Breviario D has a long standing experience in molecular biology and genetics of Eukaryotes including yeast, animals and plants. For more than 30 years he has been analyzing structural and functional features of genes and DNA genomic sequences. He has cloned several genomic and cDNA sequences including the following plant gene families : alpha- and beta-tubulin, annexin, calcium-dependent protein kinases. Rice (Oryza sativa L) has been his main plant species of interest, his main subject of research. Of recent, he has driven his attention on non coding genomic DNA sequences, particularly introns that are used in evolutionary genomic studies both as tools for establishing evolutionary distances (ILP for Intron Length Polymorphisms) and for detecting DNA polymorphisms or as tools for modulating gene expressions (IME for Intron mediated enhancement of gene expression). He is an associated Editor of Transgenic Research and acts as Referee for more than 20 International recognized plant scientific journals. He also acts as an advisor for the evaluation of National and International Grant applications among which MIUR (Italian Ministry for Research) Italian Universities ; CNR ; EMBO; BARD; NSF-USA, USDA, Newcastle University. He has been an invited speaker at both National and International Meetings and Congresses (ICGEB-UNIDO, Trieste ; NATO workshop , Kiev; International Symposium of Plant Cytoskeleton, Yalta ; AOX Symposium, Evora Portugal ; 2nd Generation Biofuels, Accra , Ghana). He has organized and chaired several National scientific events such as ; Expo dei sapori 2003-2004; Expo capitale umano e Innovazione 2006 ; Science for food-Festival della Scienza 2006, Meetmetonight 2012; Industrial Biotechnology PhD meeting 2012. He has been tutoring more than 40 different young scientists (Master students; PhD students; contractors ; fellowships). He has been teaching in several Master Courses (Managers for scientific innovation, 2000 ; Bio-communication, 2002 ; Biomasses and energy production). He has given seminars and lectures in several Foreigner Institutions (some : N.I.H Bethesda USA; E.MB.L. Heidelberg DE; Merck Sharp and Dhome ,West Point USA; NCSU Raleigh USA; CPRO-DLO, Wageningen, NL; CAAS, Beijing, China; Freiburg University, Freiburg, DE; NIAB, Cambridge, UK).
Cytoskeleton, Microtubules at a plant biology level, Gene organization (exons, introns and ncDNA sequences), evolution and expressions at a molecular biology level, leaf disease.
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