alexa
Reach Us +1-947-333-4405

GET THE APP

Analysis Of Pre-analytic Factors Affecting The Success Of Next-generation Sequencing Of Solid Tumors | 43460
ISSN: 2161-0681

Journal of Clinical & Experimental Pathology
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Recommended Conferences

World Congress on Pathology and Microbiology

Manila, Philippines
Google scholar citation report
Citations : 1147

Journal of Clinical & Experimental Pathology received 1147 citations as per google scholar report

Journal of Clinical & Experimental Pathology peer review process verified at publons
Indexed In
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Open J Gate
  • Genamics JournalSeek
  • JournalTOCs
  • Ulrich's Periodicals Directory
  • RefSeek
  • Hamdard University
  • EBSCO A-Z
  • OCLC- WorldCat
  • Publons
  • Geneva Foundation for Medical Education and Research
  • Euro Pub
Share This Page

Analysis of pre-analytic factors affecting the success of next-generation sequencing of solid tumors

5th International Conference on Pathology

Hui Chen

University of Texas MD Anderson Cancer Center, USA

ScientificTracks Abstracts: J Clin Exp Pathol

DOI: 10.4172/2161-0681.C1.019

Abstract
Clinical laboratories are rapidly implementing next-generation sequencing (NGS) tests for mutation analysis, however there are few guidelines regarding sample quality for successful results. We aimed to establish tissue quality parameters for successful NGS in solid tumors and to improve NGS performance. Using a 50-gene hotspot mutation panel we identified the major cause for unsuccessful NGS analysis being DNA <10 ng, which was associated with extremely small and low cellularity samples. High success rates were associated with resection procedures and biopsied tumor >10 mm2. Independent factors leading to lower NGS success were cellular tumor areas and decalcification procedures. Tumor type and paraffin block age did not affect success. We optimized workflow and showed improved NGS success rates with pronounced improvement among tiny samples and cytology samples. Identifying preanalytical tissue factors allows us to improve NGS performance and to successfully test tumors obtained from minimally invasive procedures.
Biography

Hui Chen has completed her MD from Peking Union Medical College, PhD from Colorado State University, Postdoctoral Research Fellowship from Johns Hopkins Medical School, Residency training from Dartmouth-Hitchcock Medical Center and Clinical Fellowship training from Memorial Sloan-Kettering Cancer Center. She is an Assistant Professor in Molecular Pathology and Breast Pathology at MD Anderson Cancer Center.

Email: [email protected]

Top