Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar
Reach Us +1-947-333-4405
GET THE APP
Association Between MiR-200c And The Survival Of Patients With Stage I Epithelial Ovarian Cancer: A Retrospective Study Of Two Independent Tumour Tissue Collections | 9242
ISSN: 2161-0681
Journal of Clinical & Experimental Pathology
Open Access
Like us on:
Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
Association between miR-200c and the survival of patients with stage I epithelial ovarian cancer: A retrospective study of two independent tumour tissue collections
2nd International Conference and Exhibition on Pathology
Background:
International Federation of Gynecology and Obstetrics stage I epithelial ovarian cancer (EOC) has a significantly
better prognosis than stage III/IV EOC, with about 80% of patients surviving at 5 years (compared with about 20% of those with
stage III/IV EOC). However, 20% of patients with stage I EOC relapse within 5 years. It is therefore crucial that the biological
properties of stage I EOCs are further elucidated. MicroRNAs (miRNAs) have shown diagnostic and prognostic potential in
stage III and IV EOCs, but the small number of patients diagnosed with stage I EOC has so far prevented an investigation of
its molecular features. We profiled miRNA expression in stage I EOC tumours to assess whether there is a miRNA signature
associated with overall and progression-free survival (PFS) in stage I EOC.
Methods:
We analysed tumour samples from 144 patients (29 of whom relapsed) with stage I EOC gathered from two independent
tumour tissue collections (A and B), both with a median follow-up of 9 years. 89 samples from tumour tissue collection A were
stratified into a training set (51 samples, 15 of which were from patients who relapsed) for miRNA signature generation, and into
a validation set (38 samples, seven of which were from patients who relapsed) for signature validation. Tumour tissue collection B
(55 samples, seven of which were from patients who relapsed) was used as an independent test set. The Cox proportional hazards
model and the log-rank test were used to assess the correlation of quantitative reverse transcription PCR (qRT-PCR)-validated
miRNAs with overall survival and PFS.
Findings:
A signature of 34 miRNAs associated with survival was generated by microarray analysis in the training set. In
both the training set and validation set, qRT-PCR analysis confirmed that 11 miRNAs (miR-214, miR-199a-3p, miR-199a-5p,
miR-145, miR-200b, miR-30a, miR-30a*, miR-30d, miR-200c, miR-20a, and miR-143) were expressed differently in relapsers
compared with non-relapsers. Three of these miRNAs (miR-200c, miR-199a-3p, miR-199a-5p) were associated with PFS, overall
survival, or both in multivariate analysis. qRT-PCR analysis in the test set confirmed the downregulation of miR-200c in relapsers
compared with non-relapsers, but not the upregulation of miR-199a-3p and miR-199a-5p. Multivariate analysis confirmed that
downregulation of miR-200c in the test set was associated with overall survival (HR 0?094, 95% CI 0?012?0?766, p=0?0272) and
PFS (0.035, 0.004?0.311; p=0.0026), independent of clinical covariates.
Interpretation:
miR-200c has potential as a predictor of survival, and is a biomarker of relapse, in stage I EOC.
Biography
Relevant Topics
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals