Characterization Of Pre-clinical Models Of Luminal B Breast Cancer In Orthotopic And Bone Metastasis Settings | 91477
Journal of Clinical & Experimental Pathology
Like us on:
Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
Luminal B breast cancer is a hormone receptor (ER and/or PR) positive and HER2 positive or negative carcinoma with high
proliferation rate and poor prognosis. Luminal B breast cancer has increased risk to relapse in incurable bone metastasis. Scarce
availability of pre-clinical luminal B type of breast cancer bone metastasis models sets a challenge for development of more efficient
treatments for luminal B breast cancer where therapeutic resistance is common. Aim of this study was to verify and compare the
ER, PR and HER2 status in luminal B type orthotopic and bone metastasis xenograft models. BT-474 (ER+, PR+, HER2+) human
breast ductal carcinoma cells were inoculated into the mammary fat pad or tibia bone marrow of female immunodeficient CIEA
NOG mice, presenting orthotopic and bone metastasis models, respectively. The orthotopic study was performed with and without
estradiol (E2) supplement and the bone metastasis study without E2 supplement. Tumor growth was followed for eight weeks and
histopathological evaluation and ER, PR and HER2 immunoperoxidase stainings were performed at endpoint. The orthotopic tumors
with E2 supplement expressed ER, PR and HER2. Without E2 supplement the tumors in the orthotopic and bone metastasis studies
were ER and HER2 positive but PR negative. Orthotopic tumors without E2 grew only to 38% of the animals, whereas 100% take rate
was observed with E2 supplement and in the bone metastasis study. This study highlights the importance of careful characterization
of pre-clinical models when developing new cancer therapies. Focus should be addressed not only to primary tumors but also to
bone metastases. The characterized orthotopic and bone metastasis models can be used to study new treatments for luminal B breast
cancer, e.g. targeting the IGF-1 or FGF signaling pathways that are known to affect treatment resistance and cell proliferation.
Anniina Luostarinen has graduated in Biomedical Sciences major from University of Turku in 2016. After upgrading her education from a Biomedical Laboratory Scientist who graduated in 2006, she has worked in Pharmatest Services, a preclinical contract research organization, concentrating in development of cancer and skeletal disease treatments. Her whole career has focused on the physiology of bone and the interaction of bone microenvironment and cancer. Particularly the bone histology as well as radiology is in her interest.