alexa Cross-reactivity And Epitope Analysis Of Anti-ginbuna CD4-1 And CD8α Monoclonal Antibodies With Lymphocytes Of Selected Cyprinid Species
ISSN: 2332-2608

Journal of Fisheries & Livestock Production
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

6th Global Summit on Aquaculture and Fisheries 2017
May 25-26, 2017 Osaka, Japan

Miyazawa Ryuichiro, Navaneethaiyer Umasuthan, Matsuura Yuta, Nakanishi Teruyuki
Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Japan
ScientificTracks Abstracts: J Fisheries Livest Prod
DOI: 10.4172/2332-2608-C1-008
Abstract
Statement of the problem: The two major subsets of T lymphocytes, helper and cytotoxic T cells, are defined by expression of CD4 or CD8 glycoproteins, respectively. Activation of CD4 T cells leads to their proliferation and differentiation into effector or regulatory cells that mediate or control the immunity; whereas, CD8 T cells destroy virus-infected cells as cytotoxic T cells. Both are essential for protecting the host from pathogens. Therefore, it is important to understand the function of CD4 and CD8 T cells. However, analysis of fish immune mechanisms has been hampered by the lack of suitable tools such as monoclonal antibodies (mAbs) against CD4+ and CD8+ T cells. Methodology & Theoretical Orientation: We have generated mAbs against CD4-1 and CD8α in ginbuna crucian carp. In this study, we analyzed the cross-reactivity of these antibodies against the lymphocytes from eleven cyprinid species by flow cytometry. According to the reactivity of antibodies, we categorized them into high-, medium- and low-reactivity groups. Additionally, we cloned the ORFs of CD4 and CD8 and analyzed their protein sequences from each fish. Findings: We identified those lymphocytes from four fish species cross-reacted with ginbuna CD4-1 mAb and lymphocytes from seven species cross-reacted with ginbuna CD8α mAb. High-reactivity group shared similar sequence characteristics with ginbuna CD4-1 and CD8α, especially in terms of the candidate epitopes of antibodies. By comparing the sequences of each groups, we identified the potential candidate epitopes, including a few epitopes for CD4-1 mAb, and one epitope for CD8α mAb. Conclusion & Significance: The epitopes of our antibodies have been well-conserved in examined cyprinid species. Our antibodies will be available for analysis of the immune mechanisms in cyprinid fish. Furthermore, present strategy can be applied to predict the epitope recognized by antibodies in other fish species than cyprinid.
Biography

Ryuichiro Miyazawa, the second year student of the Doctor course, Graduate School of Veterinary Medicine, Nihon University. He has been involved in the studies on fish immune mechanisms using zebra fish and ginbuna as model fish. Recently, he found that monoclonal antibodies against ginbuna CD4-1 and CD8α cross react with lymphocytes of zebra fish and other cyprinid fishes. He is interested in T cell function in fish and investigates the autoimmune mechanisms from an evolutionary perspective at present. Monoclonal antibodies against T cell subsets are indeed quite helpful to analyze T cell function in zebra fish suffering from autoimmune disease. He has published four papers in international journals including Miyazawa et al., Dev. Comp. Immunol, 2016. He presented six papers at international conferences and domestic meetings.

Email: [email protected]

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords