Reach Us +1-947-333-4405


Cytotoxic Effects Of Diosgenin On Human Squamous Cell Carcinoma Cell Line: An In Vitro Study | 9204
ISSN: 2161-0681

Journal of Clinical & Experimental Pathology
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Google Scholar citation report
Citations : 1174

Journal of Clinical & Experimental Pathology received 1174 citations as per Google Scholar report

Journal of Clinical & Experimental Pathology peer review process verified at publons
Indexed In
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Open J Gate
  • Genamics JournalSeek
  • JournalTOCs
  • Ulrich's Periodicals Directory
  • RefSeek
  • Hamdard University
  • OCLC- WorldCat
  • Publons
  • Geneva Foundation for Medical Education and Research
  • Euro Pub
Share This Page

Cytotoxic effects of diosgenin on human squamous cell carcinoma cell line: An in vitro study

2nd International Conference and Exhibition on Pathology

Ehab S. Abd Elhamid

ScientificTracks Abstracts: J Clin Exp Pathol

DOI: 10.4172/2161-0681.S1.008

Background: Diosgenin, a furostanol saponin, is a major bioactive constituent in the seeds of fenugreek. Extracts of fenugreek seeds and some of their saponin constituents have been found to have anticarcinogenic potency in different settings. Aims and Objectives: Estimate the anticancer effect of Diosgenin on squamous cell carcinoma cell line. Methods: The HEp-2 human laryngocarcinoma cell line was utilized. Hep-2 cells were treated using 20, 40 and 60μM of diosgenin for 6, 18, 24 and 48 hours. The surface area and circularity of the nuclei were automatically measured and nuclear area factor (NAF) was calculated. The mean values of (NAF) of different concentrations compared to the control results at different intervals were assessed statistically. The DNA fragmentation assay was performed to monitor the effect of diosgenin treatment relative to time and concentration on DNA pattern. Results: The cytotoxic effect of diosgenin on HEp-2 cells was in direct relationship with the concentration of diosgenin and the time of exposure. The mean NAF decreased progressively from 6 hours till 24 hours with different diosgenin concentrations but it showed an increase as the incubation time increased to 48 hours. With 20μM diosgenin concentration, the mean value of NAF decreased as the treatment time increased from 6 to 48 hours. The DNA laddering showed more obvious DNA fragmentation with diosgenin concentrations of 40μM and 60μM than 20μM. Conclusions: The 40μM and 60μM diosgenin concentration caused an increase in the incidence of apoptotic criteria. However, 40μM diosgenin concentration after 48 hours showed more apoptotic changes than that observed with 60μM as evidenced by morphometric analysis.