Effects Of Fractionated Methanolic Leaf Extract Of Gongronema Latifoliumon CCl4-induced Wistar Albino Rats | 92083
Journal of Clinical & Experimental Pathology
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The general aim of this study is to assess the effects of fractionated methanolic leaf extract of Gongronema latifoliumon
CCl4-induced wistar albino rats. Fifty-four wistar albino rats were divided into seven treatment groups. Group A was
given feed and water, Group B was injected with olive oil intraperitoneally, while the rest of the groups (C, D, E, F and G) were
injected intraperitoneally with a single dose of CCl4 (148 mg/kg). After 36 hours of induction, group E, F and G were given 100
mg/kg, 150 mg/kg and 200 mg/kg body weight of n-butanol fraction of methanol leaf extract of Gongronema latifolium by oral
gavage. Group D was given 100 mg/kg of silymarin (standard drug) where as group C served as CCl4-induced group. At the
end of 28 days of treatment, there were significant (P<0.05) reduction in PCV, Hb concentration and serum protein levels as
well as a significant (P<0.05) increase in percentage change in liver weights of CCl4-induced control rats when compared with
the induced treated groups. Liver marker enzymes studies showed that there was significant (P<0.05) increase in the serum
activities of ALT, AST, ALP and bilirubin concentrations in CCl4-induced control group when compared with the induced
treated groups. Antioxidant assay on the liver homogenate showed that there was significant (P<0.05) decrease in SOD, CAT,
GPx and a significant increase (P<0.05) in MDA of CCl4-induced control rats when compared to the induced treated and
normal control groups. These findings suggested that n-butanol fraction of methanol leaf extract of G. latifolium may have
anti-hepatotoxic and antioxidative effects against CCl4-induced liver damage rats.
Okpala, Jude Chinedu is currently a PhD student at the Department of Biochemistry, Ahmadu Bello University where he also did his Masters Degree programme. He also attended Ebonyi State University, Abakaliki where he bagged his Bachelor of Science Degree. He is currently an independent researcher and has published several papers in reputed journals. He has also served as an editorial board member of several journals.