Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar
GET THE APP
Enteropathogenic Yersinia Frederiksenii- Contained A Novel MsDNA-Yf79 (Multicopy Single-Stranded DNA) With The Genomic Analysis Of It?s Retron | 3149
Journal of Clinical & Experimental Pathology
Like us on:
Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
Yersinia frederiksenii is a Gram negative enteropathogenic bacterium and is reclassified from Yersinia enterocolitica. It causes
enterocolitis with other diseases in human. On the other hand, retron DNAs are commonly associated with prophage DNA
and are found in the genomes of a wide variety of different pathogenic bacteria like in (Vibrio cholerae, Vibrio parahaemolyticus,
Salmonella typhimurium, Escherichia coli species and also in other pathogenic bacteria). The retron reverse transcriptase (RT)
is used to synthesize a strange msDNA (multicopy single-stranded DNA). This msDNA is a satellite DNA and is composed
of a small, single-stranded DNA, linked to a small, single-stranded RNA molecule by a unique 2?, 5?- Phosphodiester bond
which is synthesized by the bacterial reverse transcriptase (RT). The present study was undertaken to investigate whether the
enteropathogenic Yersinia frederiksenii may contain such type of novel retron element in their genome sequences.
This study has assigned the retron-Yf79 in Yersinia frederiksenii ATCC 33641 Contig01029, together with its codon bias
and G+C content which have analyzed by using Bioinformatics tools. The region (retron-Yf79) required for the biosynthesis of a
branched RNA-linked msDNA-Yf79 and the element consist of a unique 2.8 kilo base sequence, contain genes for msDNA-RNA
(msd,msr), a gene (Yfred0001_42830) for reverse transcriptase (RT) and a gene (Yfred0001_42840) for an open reading frame
(ORF-541), consisting of 541 amino acids. The conserved guanine (G) residue at position 12 in RNA part of msDNA-Yf79 has
also been found in msDNA-RNA complexes and is essential for branch formation. The RT-Yf79 (reverse transcriptase in Yersinia
frederiksenii) and open reading frame-541 (ORF-541) genes used AAA codon for lysine with a frequency of 55% and 74%,
respectively whereas Yersinia frederiksenii genome itself only used AAA codon for lysine with a frequency of 20% of the time.
Subsequently we perceived that, the (G+C) content of RT-Yf79 and (G+C) content of open reading frame-541 (ORF-541) genes
was same (41%) indicating that, the open reading frame-541 (ORF-541) was a member of this retron (retron-Yf79) element.
Whereas, the general (G+C) content of the Yersinia frederiksenii ATCC 33641 contig01029 genome was 45 %. The codon bias,
G+C content, phylogenetic analysis of reverse transcriptase (RTs) and also the 16S rRNA from pathogenic bacteria revealed that,
this retron (retron-Yf79) was a foreign DNA element. It might have integrated at once upon a time during their evolution through
the vertical descent rather than the horizontal transformations by a mechanism related to phage recognition and transposition.
Because, it contains a transposase gene located just downstream of retron-Yf79 element which might participate in transposition
of this novel retronelement. All of these results document evidence, in support of the importance of retron element towards the
expression of pathogenic potential of Yersinia frederiksenii.
Mr. Rasel Das has completed my MS in Biotechnology at the age of 24 years from the University of Science and Technology Chittagong (USTC),
Bangladesh. Now I am doing my job as Research Assistant and Lecturer at the Department of Biochemistry and Biotechnology (USTC). This work
is published in Journal of Pathogen (UK) in their special issue in 2011.
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals