Occupational Medicine & Health Affairs
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Green alga Scenedesmus quadricauda was used in our study as a tool for screening of clodinafop-propargyl resistance in
plants. Cultures of alga were treated with different concentrations of the herbicide (0, 10.5, 21, 42 and 84 ppm). Allelespecific
PCR assay used to monitor resistance alleles in algae and total protein analysis showed inducing or inhibition of
synthesis of some polypeptide fractions by the treatments depending upon herbicide concentration. In a transmission electron
microscopy investigation, longitudinal sections in algal cell wall showed clear response to different concentrations of the
herbicide. This response was demonstrated as change in cell shape and increase in cell size as well as thickness in the layers
forming cell wall. The cell number, free amino acid content, chlorophyll ?a?, ?b? and caroteniods determined after 0, 24, 48, 72
and 96 hrs for six generations, indicated the possibility of using the green alga S. quadricauda cell suspension for screening of
herbicide resistance as a simple tool in environmental impact assessment of herbicides.
Sayed Ashour Ahmed has completed his PhD at the age of 35 years from Weed Science Laboratory, Faculty of Agriculture, Kyoto University, Japan. He is an
Emeritus Professor at Plant Protection Dept of Faculty of Agriculture, Assiut University, Egypt. After 45 years working (research and education) he has published
more than 60 research and working papers in international and local journals and conferences in the field of environmental and pesticide science.
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