Journal of Fisheries & Livestock Production
Open Access

Abstract

Improvement of genetic qualities in Bali cattle can be done by selection based on genetic marker. MSTN (Myostatin) gene is known as an inhibitor or negative regulator of muscles development in embryogenesis and myogenesis. Mutation of this gene caused double muscling characteristic in cattle and it was identified has significant associated with some growth traits in cattle, sheep, pigs and mice. A total 48 Bali cattle from Bali cattle breeding centre was screened to identified genetic polymorphisms in MSTN promoter region by using direct-sequencing method (GenBank: AF348479.1). The weights and bodies measurement of Bali cattle were collected at 12 months. The muscling traits were evaluated by using ultrasound veterinary scanner at frequency 6.5 Hz and 130 mm of deep. The polymorphic SNPs in this region were 23 SNPs (g.-8495C>T, g.-8455A>C, g.-8444G>A, g.-8428A>G, g.8361G>A, g.-8350A>G, g.- 8313A>G, g.-8254T>G, g.-8223C>T, g.-8313A>G, g.-8.184C>A, g.-8173A>G, g.-8161C>T, g.-8158C>A, g.-8144T>C, g.-8141G>C, g.-8124T>C, g.-8098C>T, g.-8087C>G, g.-8086C>T. Based on statistical analyses, SNPs g.-8444G>A, g.-8428A>G, g.8361G>A, g.- 8313A>G and g.-8313A>G were significantly associated with ultrasound back fat thickness. SNP g.-8.184C>A and g.-8087C>G was significantly associated with ultrasound rump thickness and ultrasound longissimus dorsi thickness (P≤0.05) respectively. This result showed that SNPs in MSTN gene could be suggested as good marker for muscling traits in Bali cattle.

Biography

Email: himmatulk@gmail.com