alexa
Reach Us +1-845-458-6882

GET THE APP

Molecular Profiling Of Papillary Thyroid Cancer Identifies Functional Biomarkers | 12392
ISSN: 2161-119X

Otolaryngology: Open Access
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Recommended Conferences

7th European Otolaryngology-ENT Surgery Conference

Vienna, Austria
Google scholar citation report
Citations : 560

Otolaryngology: Open Access received 560 citations as per google scholar report

Otolaryngology: Open Access peer review process verified at publons
Indexed In
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Open J Gate
  • Genamics JournalSeek
  • RefSeek
  • Hamdard University
  • EBSCO A-Z
  • OCLC- WorldCat
  • Publons
  • Geneva Foundation for Medical Education and Research
Share This Page

Molecular profiling of papillary thyroid cancer identifies functional biomarkers

International Conference and Exhibition on Otolaryngology

Jan Geliebter

ScientificTracks Abstracts: Otolaryngology

DOI: 10.4172/2161-119X.S1.002

Abstract
The ACS estimates that there will be ~56,000 new cases of thyroid cancer in 2012, approximately 80% of which will be papillary thyroid carcinomas (PTC), an increasing number since 1980. Molecular profiling of PTC offers a valuable tool for identifying genes that can be used as diagnostic and prognostic markers. Molecular profiling of PTC samples and paired controls identified 177 differentially expressed genes (98 upregulated and 79 downregulated). The upregulation of potential biomarkers was verified in expanded sample sets by qRT-PCR and immunohistochemisty, and included arachidonic acid 5-lipoxygenase (ALOX5), urokinase plasminogen activator and its receptor (uPA/uPAR) and kallikreins 7 and 10 (KLK7/KLK10). ALOX5 expression was elevated in PTC (22.57 fold) and demonstrated a direct correlation with tumor invasiveness. Transfection of ALOX5 into the PTC cell line, BCPAP, resulted in increased collagenase activity, MMP- 9 secretion and invasiveness. Inhibitors of MMP-9 and ALOX5 reversed the ALOX5-enhanced invasion. uPA and uPAR transcription was increased in PTC (2.4 and 10.1 fold, respectively) and in BCPAP, relative to a normal thyroid cell line. siRNA-mediated downregulation of uPAR in BCPAP cells resulted in decreased activity in the FAK/PI3K/Akt signaling pathway, proliferation, clonigenicity and migration/ invasion. KLK7 and KLK10 RNA expression in PTC was upregulated (21.0 and 22.0 fold) and immunohistochemical analysis on patient samples revealed increased and coordinated expression. These data provide new evidence of ALOX5 and uPA/uPAR system´┐Żs functional roles in PTC invasion/metastasis, and demonstrate their attractiveness as molecular biomarkers and therapeutic targets. The role of KLKs in PTC pathogenesis is currently under investigation.
Biography

Dr. Geliebter received his PhD from SUNY, Downstate Medical Center and completed postdoctoral research at The Albert Einstein College of Medicine, Bronx, NY. He has held faculty positions at The Rockefeller University and the Albert Einstein College of Medicine and he is currently a Professor in the Departments of Microbiology & Immunology and Otolaryngology at New York Medical College and the Resident Research Coordinator at New York Eye and Ear Infirmary. He has published more than 60 papers in peer-reviewed journals in the fields of thyroid and prostate cancer and molecular genetics of the immune system.

Top