alexa Mr-Masc Gene For Sex-regulation And Production Of All-male Populations In The Giant Freshwater Prawn Macrobrachium Rosenbergii
ISSN: 2332-2608

Journal of Fisheries & Livestock Production
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

6th Global Summit on Aquaculture and Fisheries 2017
May 25-26, 2017 Osaka, Japan

Wen-ming Ma, Dong-rui Li, MS and Wei-jun Yang
Zhejiang Wanli University, China
ScientificTracks Abstracts: J Fisheries Livest Prod
DOI: 10.4172/2332-2608-C1-008
Abstract
Statement of the Background: Macrobrachium rosenbergii is one of the most important prawns in freshwater aquaculture. Male giant freshwater prawns grow faster and reach higher weights at harvest than females, which make the culture of all-male populations desirable. The purpose of this study is to explore the molecular information of new gene and its potential physiological function on sex regulation. Methodology & Theoretical Orientation: The gene expression pattern was studied using Real-time RT-PCR. The functional role of gene in vivo by RNA interference (RNAi) was utilized and its effect on sex regulation was evaluated. Findings: In this study, a novel gene of the full-length cDNA encoding a masculinizing factor from the giant freshwater prawn Macrobrachium rosenbergii was termed Mr-Masc. Real-time RT-PCR analysis revealed that Mr-Masc gene expression was obviously difference between male and female prawn, and dominantly expressed in the ovary of the reproductive tract in female prawn. To elucidate the functional role of Mr-Masc in vivo, the Mr-Masc gene was disrupted by RNA interference (RNAi). In vivo silencing of the gene, by injecting the all-male and monosex postlarvae with Mr-Masc double-stranded RNA, sex-revsersed females (neofemales) were obtained. Moreover, all-male progeny was produced by mating neofemales with normal males and these all-male populations showed good growth potential and market value in the aquaculture. Conclusion & Significance: It was firstly reported the full and functional sex reversal of male freshwater prawns through the silencing of a single Mr-Masc encoding gene. Neofemales capable of mating and spawning were produced by the RNAi technology at a sufficiently early stage of larval development. This finding offered an insight regarding the biology and evolution of sex differentiation regulation. Since sexual dimorphic growth patterns are common among decapod crustaceans, it is obvious that the results of this study will have applied significance for many aqua cultured species.
Biography

Wen-ming Ma has expertise in sex determination and sex differentiation of the marine shrimp and prawn, functional gene discovery and application research. Meanwhile, make a good progress in sex control and all-male unisexual cultivation in Macrobrachium rosenbergii.

Email: [email protected]

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords