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Quantitative Fuculokinase Gene Polymerase Chain Reaction For Specific Detection Of Haemophilus Influenzae Pneumonia | 9243
ISSN: 2161-0681

Journal of Clinical & Experimental Pathology
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2nd International Conference and Exhibition on Pathology

Guma M. K. Abdeldaim, Kristoffer Stralin, Per Olcen, Jonas Blomberg, Paula Molling and Bjorn Herrmann
Accepted Abstracts: J Clin Exp Pathol
DOI: 10.4172/2161-0681.S1.010
Abstract
Aquantitative polymerase chain reaction (PCR) for the fuculokinase gene ( fucK ) was developed for specific detection of Haemophilus influenzae . The method was tested on sputum and nasopharyngeal aspirate (NPA) from 78 patients with community-acquired pneumonia (CAP). With a reference standard of sputum culture and/or serology against the patient's own nasopharyngeal isolate, H. influenzae etiology was detected in 20 patients. Compared with the reference standard, fucK PCR (using the detection limit 105 DNA copies/mL) on sputum and NPA showed a sensitivity of 95.0% (19/20) in both cases, and specificities of 87.9% (51/58) and 89.5% (52/58), respectively. In a receiver operating characteristic curve analysis, sputum fucK PCR was found to be significantly superior to sputum P6 PCR for detection of H. influenzae CAP. NPA fucK PCR was positive in 3 of 54 adult controls without respiratory symptoms. In conclusion, quantitative fucK real-time PCR provides a sensitive and specific identification of H. influenzae in respiratory secretions.
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