Transcriptome Profiling For Identifying Genes To Develop Abiotic Stress Tolerant Transgenic Wheat | 105042
Advances in Crop Science and Technology
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Wheat (Triticum sp.) stands as the second largest staple crop of the world with 17% of the total cultivatable land under wheat
production. Global annual wheat production needs to be increased at quantum leaps from the present production of more
than 650 million metric tons so as to feed the ever burgeoning world population. Unfortunately, with the changing global climate,
various abiotic stresses further hamper the wheat productivity. Development of abiotic stress tolerant cultivars is necessary to achieve
the goal of enhanced wheat productivity. With the available gene pool within a species becoming limited, it becomes imperative that
we search genes responsible for abiotic stress tolerance across the, genus, species and even kingdom and using rDNA technology
develop transgenic wheat tolerant to abiotic stresses. The present study involved identification of abiotic stress responsive genes from
tolerant plant systems as Penniseteum glaucum, Triticum aestivum, Ziziphus nummularia and Prosopis cineraria using Roche 454 and
Illumina sequencing platforms. De novo assembly and transcriptome annotations were preformed to have insight about genes, gene
family and transcriptional factors related to abiotic stress. Further analysis for change in expression level of known and unknown
genes, SNP detection and SSR marker detection have been carried out to identify stress responsive genes and stress tolerance linked
markers. A few stress responsive genes as NAC, P5CS, WRKY, HSP, MYB, ASR, DREB etc have been identified and characterized.
These genes are further functionally validated and have been transformed in elite Indian bread wheat for development of transgenic
wheat tolerant to abiotic stress.
Jasdeep Chatrath Padaria has completed her PhD from Indian Agricultural Research Institute and Postdoctoral studies in the area of Gene expression profiling with respect to abiotic stress tolerance at Department of Horticulture and Landscape Architecture, Purdue University, USA. She is working as a Principal Scientist in the area of Biotechnology and Climate Change at National Research Centre on Plant Biotechnology, a premier institute in the area of plant molecular biology and biotechnology. She has published more than 25 papers in reputed journals and has guided 15 MSc and PhD students.