Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar
Wnt5a Expression Affects The Biological Behavior Of HCC | 3157
Journal of Clinical & Experimental Pathology
Like us on:
Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
Wnt5a has been shown to be involved in cancer progression in a variety of tumor types and described as both challenges
the simplistic classification of gene as tumor promoters or suppressors. Our previous experimental studies have indicated that
Wnt5a commands a tumor-suppressing effect and it was shown to be downregulated in hepatocellualar carcinomas. In this study
we designed to explore the effects of Wnt5a gene on HCC cell line.
Human hepatocellular cells of the line Huh7 were transfected with pcDNA3.1-Wnt5a or pcDNA3.1 contruct. The
integration of the plasmid DNA and the expression of Wnt5a were confirmed by RT-PCR and Western Blot respectively. Plate
colone formation test to calculate the clone formation rate and cell cycle was analyzed by flow cytometry. Scratch assays and
Xenograft studies in nude mice were used to examine cell migration. Using Western Blot detect the associated protein of Wnt5a,
Ror2, E-cadherin and p53, expression of Huh7 cells.
FCM analysis showed that The G1 and S phase fraction in Huh7 cells with Wnt5a expression vector transfected were
64.92%?2.97% and 21.69%?3.27%, compared with emoty vector transfected cells in S phase fraction decreased but increased
in G1 phase fration(57.80%?1.09% and 29.03%?1.32%, all p<0.05), suggested pcDNA3.1-Wnt5a transfection suppressed the
progression of G1→S phase in Huh7cell cycle. The clone formation rates of pcDNA3.1 group was 16.47%?0.39%, significantly
higher than Wnt5a expression group(8.07%?0.37%, P<0.01)in the Huh7 cells. The result of scratches assay indicated that scratch
after 48 hours, the cells with pcDNA3.1 transfected of the scratch area boundary was not clear and the cells in scrath zone
were significantly increased than Huh7 cells with pcDNA3.1-Wnt5a transfected. Wnt5a expression resulted in suppressing the
migration of Huh7 cells. In Xenograft studies in nude mice we found that: tumor volumes were significantly decreased in nude
mice injected with pcDNA3.1-Wnt5a transfected Huh7 cells compared with null vector pcDNA3.1. Expression of Wnt5a, Ror2
and E-cadherin protein were increased in Huh7 cells which transfected pcDNA3.1-Wnt5a compared with Huh7 cells transfected
the null vector. But P53 protein was expression at about the same level in two groups.
Wnt5a expression resulted in suppression cell proliferation and migration. Wnt5a may be a tumor suppressor gene
in HCC through Ror2, receptor for Wnt5a, to activate non-canonical Wnt signaling pathway.
Professor Geng M, the director of the Department of Pathology, General Hospital of Jinan Military Command, has completed his M.D. at the age of
23 years from The Fourth Military Medical University. He is good at the area about pathological diagnosis and research of gastrointestinal tumor. He
has published more than 30 papers in reputed journals and serving as an editorial board member of repute. Research projects were supported by
the national and provincial natural science funds, and research achievements got awards from the Shandong province and the Army.
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals