Journal of Bioremediation & Biodegradation
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Lignocellulosic bioethanol production now-a-days is gaining increasing interest due to global warming, hike in oil price etc.
But there are several technological and other challenges associated with bioethanol production. Technological challenges are
development of efficient pretreatment step which can significantly degrade lignin without altering carbohydrates, efficient hydrolysis
step and development of fermentation step which can utilize both pentose and hexose sugars. The present study has focused on
bioprocess development for bioethanol production from a mixture of food wastes (spinach, cabbage, peels of onion and orange). First
liquid hot water (LHW) pretreatment of food waste was optimized by varying different parameters (temperature, incubation time
and substrate concentration). Maximum reducing sugar yield (525.60 mg/gram dry substrate) was found at substrate concentration
10% (w/v), temperature 160 oC and incubation time 30 min. After optimization, LHW pretreated biomass was characterized using
Fourier transformed infrared spectroscopy (FTIR), X-ray diffraction (XRD), Scanning electron microscopy (SEM) and biochemical
composition analysis. Further, pretreated biomass was hydrolysed using whole cells of Fusarium incarnatum KU377454 (locally
isolated strain) without addition of any enzymes. It showed maximum reducing sugar yield of 580.95 mg/gram dry substrate) within
3 days of incubation at 30 oC. The produced sugar hydrolysate was further fermented using co-cultures of hexose fermenting strain
(Sacchromyces cerevisiae) and pentose fermenting strain (F. oxysporum). Maximum ethanol production (3.25%, v/v) was observed
after 48 h of incubation at 35 oC. The present study, reports development of efficient thermal pretreatment without addition of any
chemicals. Further carbohydrates, part of pretreated biomass were converted into reducing sugars by whole fungal strain without the
use of any costly chemicals. Lastly, fermentation process was optimized using co-culture strategy which yielded maximum ethanol
from both pentose and hexose sugars. This study can be useful for commercial bioethanol production from food waste.
1. Sharma S, Sharma V, Kuila A (2018) Simultaneous saccharification and fermentation of corn husk by co-culture strategy.
Journal of Petroleum & Environmental Biotechnology (Revised manuscript under review).
2. Sharma S, Sharma V, Kuila A (2018) Thermochemical pretreatment of corn husk and enzymatic hydrolysis using mixture
of different cellulases. Biomass Conversion and Biorefinery, 8:179-188.
3. Madhawan A, Aroora A, Das J, Sharma S, Kuila A, Sharma V (2017) Different types of thermochemical pretreatment and
optimization of enzymatic hydrolysis of groundnut shell. Waste and Biomass Valorization (Accepted), doi: 10.1007/s12649-
4. Sharma S, Kuila A, Sharma V (2017) Enzymatic hydrolysis of thermochemically pretreated biomass using a mixture of
cellulolytic enzymes produced from different fungal sources. Clean Technologies and Environmental Policy, 19:1577-1584.
5. Madhawan A, Aroora A, Das J, Kuila A, Sharma V (2017) Microreactor technology for biodiesel production: a review.
Biomass Conversion and Biorefinery (Accepted), doi: 10.1007/s13399-017-0296-0.
Dr. Vinay Sharma, currently Professor and Head (Chair), Department of Bioscience and Biotechnology at Banasthali Vidyapith has over 35 years experience of teaching and research in Plant Sciences/ Biotechnlology at I.I.T. Roorkee earlier and presently at Banasthali. He has delivered over 100 invited/ keynote lectures and has chaired sessions at many national and international fora in India and abroad. He had extensive international research experience as Postdoc/ Visiting Professor at many institutions including Max Planck Institute, Koeln, Technical University, Darmstadt, Germany, University of Central Florida, USA and others. He has published over 300 research papers, has authored 6 books and has mentered 55 doctoral students. He has been honoured with several prestigious fellowships and awards in India and abroad including Fellow of National Academy of Sciences. He has keen interest in Plant Biology (Plant Stress/ Plant Informatics)/ Biotechnology and his current major research focus is on Biofuels (lignocellulosic bioethanol/ biodiesel).