Development Of Stable Recombinant Cyanobacteria For Economically Competent Solar-fuel-factories: Ethylene Production | 58003
Journal of Bioremediation & Biodegradation
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Greenhouse gas emissions and limited fossil fuel reserves increase the need to find alternative ways to generate substitutes
for petroleum-derived products such as ethylene. Ethylene is a simple alkene of commercial value due to multitude of
large-scale uses in plastic industry and ever growing demand. One of the promising approaches is to use cyanobacterial cells as
biological factories, through their photosynthetic capacity to produce ethylene using atmospheric CO2 and water as substrates.
The biosynthesis of ethylene has been studied in Synechococcus sp 7942 by over-expressing the heterologous ethylene forming
enzyme (efe) from Pseudomonas syringae which converts the endogenous metabolic precursor 2-oxoglutarate to ethylene. As
a volatile gas, ethylene then diffuses out from the cell and spontaneously separates into the culture headspace for collection
and analysis. We have studied different aspects of observed genetic instability which have earlier compromised prolonged
ethylene production in Synechococcus, and have developed stable production strains capable of sustained autotrophic
ethylene biosynthesis. Although the production levels still remain below the threshold required for commercial applications,
cyanobacteria have been intensively studied in this respect, and a range of molecular biology tools and production platforms
are being developed and characterized.
Veronica Carbonell is Licentiate on Environmental Sciences by the University of Miguel Hernandez with strong international background and has participated in international programs such as Erasmus at Free University of Brussels (Belgium), FARO at John Innes Center (UK) and IAESTE at Silesian University of Technology (Poland). Currently, she is pursuing her PhD from the University of Turku, Finland.