alexa Effects Of NF-κB Inhibitor On The Regulation Function Of Cigarette Smoke Extract To Human β Defensins In The Immortalized Human Oral Mucosal Epithelial Cell Line
ISSN: 2332-0702

Journal of Oral Hygiene & Health
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

2nd International Conference on Restorative Dentistry and Prosthodontics
May 01-02, 2017 Toronto, Canada

Qian Yajie
Nanjing University, China
Posters & Accepted Abstracts: J Oral Hyg Health
DOI: 10.4172/2332-0702-C1-006
Abstract
Cigarette smoke increases the susceptibility to oral mucosal infection and is a risk factor for malignant transformation. Cigarette smoke is a mixture of thousands of toxic components generated upon the burning or heating of tobacco leaves. The toxic components first interface with the immune system at the oral mucosal surfaces. NF-κB is a new member of the family of transcription factors, which positively regulate the expression of many genes involved in inflammatory and other responses, including human β defensins (hBDs). The hBD family is one type of cationic antimicrobial peptides that can be secreted by epithelial cells. Among hBD family, hBD-1, -2 and -3 are critical members of the defense system of oral mucosal epithelium. We treated immortalized human oral mucosal epithelial (Leuk-1) cells with various concentrations of cigarette smoke extract (CSE) for 24 h. Western blotting and immunofluorescence assays were performed to study CSE-induced alteration of NF-κB, and P-NF-κB protein expression. The change of hBDs expression were tested using qPCR and ELISA. And then we adopted BAY 11-7082, a specific inhibitors of NF-kB, to inhibit the activation of NF-kB signalling. Different concentrations of NF-κB inhibitor NF BAY 11-7082 were treated to Leuk-1. Then 10 μM BAY 11-7082 was added to the cell culture 24 h before the addition of 4% CSE for 24 h. Leuk-1 cells were treated with 0.5% DMSO as a mock-treated control. Real-time PCR and ELISA were performed to detect the mRNA levels and secretion of hBD-1, -2, and -3, respectively. In this research, we found CSE treatment suppressed NF-κB expression and activated P-NF-κB expression in Leuk-1 cells. The mRNA and secretory levels of hBD-1 and -3 were down-regulated by CSE, while the mRNA and secretory level of hBD-2 were up-regulated by CSE. The BAY 11-7082 treatment significantly abrogated the inhibitory effect of CSE on hBD-1 mRNA expression and release, BAY 11-7082 treatment remarkably reversed the induced effect of CSE on hBD-2 mRNA level and release, while BAY 11-7082 treatment clearly removed the inhibitory effect of CSE on hBD-3 mRNA level and release. The present study indicated that CSE regulated the expression levels of hBDs via down-regulated NF-κB in oral mucosal epithelial cells.
Biography

Qian Yajie completed her Master’s degree from Nanjing University. She is now a Clinical Doctor at Nanjing Stomatological Hospital Medical School of Nanjing University. Since the beginning of the graduate student stage, she has been engaged in Oral Medicine research. So far, she has published a paper as the first author, in Cellular Physiology and Biochemistry. She contributed in the other three papers, which were all included in SCI. In recent years, she has participated in several research projects, and won a number of awards, including “New Technological Introduction Award” from the Health Department of Jiangsu Province in 2014.

Email: qian_qq@163.com

image PDF   |   image HTML
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

agrifoodaquavet@omicsonline.com

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

clinical_biochem@omicsonline.com

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

business@omicsonline.com

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

chemicaleng_chemistry@omicsonline.com

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

environmentalsci@omicsonline.com

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

engineering@omicsonline.com

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

generalsci_healthcare@omicsonline.com

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

genetics_molbio@omicsonline.com

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

immuno_microbio@omicsonline.com

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

omics@omicsonline.com

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

materialsci@omicsonline.com

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

mathematics_physics@omicsonline.com

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

medical@omicsonline.com

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

neuro_psychology@omicsonline.com

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

pharma@omicsonline.com

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

social_politicalsci@omicsonline.com

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version