TLR4 Gene Polymorphism And Inflammatory Response In Chronic Periodontitis | 103108
Journal of Oral Hygiene & Health
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Statement of the Problem: periodontal disease is one of the most common chronic diseases in humans and is induced by
microbial pathogens that reside in the oral cavity. The Toll-like receptor (TLR) family
of innate immune recognition receptors plays a fundamental role in the induction of
innate immunity, inflammation, cell survival, and proliferation. The presence of TLR4
has been shown to be essential for the progression of inflammation and related to
the bone metabolism in periodontitis Pelargonium sidoides DC. root extract (PSRE)
demonstrated possibility to exert stimulatory effects on the innate immune system.
Purpose of this study: determine the prevalence of polymorphism of TLR4 rs1927911
gene in patients with periodontitis in association with clinical signs of periodontitis and
assess the protective effect of Pelargonium sidoides DC root extract (PSRE).
Methods: data from 40 patients with periodontitis, treated with PSRE and 20 subjects
of the control group were studied. DNA extraction from patients??? peripheral blood
leukocytes using commercial DNA extraction kits was performed. Genotyping using
a real-time polymerase chain reaction method was done. Statistical data analysis using
IBM SPSS Statistics program was done.
Results: the frequency of TLR4 rs1927911 (C/T) genotypes and alleles in patients with
periodontitis and control group subjects was evaluated. Patients with periodontitis: 9.1% have TT, 39.4% - TC , 51.5% - CC
genotype and 28.8% have T allele and 71.2% C allele; respectively in control group: 6.3%, 36.5% , 57.1%, 24.6% and 75.4%. No
statistically significant differences, of all the studied genotype and allele frequencies, between periodontitis and control group
were found. The impact of polymorphism of the TLR4 rs1927911 gene, correlation between polymorphism and susceptibility
to periodontitis were evaluated and no statistically significant differences were found.
Conclusions: There was no statistically significant difference between TLR4, genotypic and allelic frequencies in patients with
periodontitis and those in the control group. Continuing of TLR4 gene polymorphism studies by increasing the number of
patients with periodontitis would be advisable. It could reveal the significance of genetic and environmental factors for the
onset of the disease more. Polymorphisms of TLR 4 gene could be used as molecular markers for the prediction of the disease.
Alina Smalinskiene is Scientific Researcher at the laboratory of Molecular Cardiology of Institute of Cardiology, Lithuania Lithuanian University of Health Sciences and Associate professor in the Lithuania Lithuanian University of Health Sciences. A.Smalinskiene received her undergraduate training in Biology at Vilnius University at Nature Faculty and the Ph. D. degree in Biology from Kaunas University of Medicine. A. Smalinskiene has accumulated research experience of genetic epidemiological lipid metabolism disorders. A. Smalinskiene was a supervisor of project funded by the State studies and science foundation and economic entity. 2010-2 014 she worked on three Research Council of Lithuania projects. She is an author and co-author of 31 scientific articles. A. Smalinskiene also participated in 60 conferences, where she presented the results of her work. She participates in the teaching of genetics and cell biology to graduate students.