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Among many genera of free living Amoebae, Acanthamoeba spp arethe causative agent of diseases such as keratitis and
encephalitis. Presence of pathogenic Acanthamoeba in clinical settings such as hospitals could be a health threat for
patients and high-risk people. The aim of the present study was to screen for the presence of Acanthamoeba in dust and biofilm
samples in clinical settings located in Tehran, using culturing and molecular approaches. A total of 112 dust and biofilm samples
from wards of clinical settings were collected and examined for the presence of Acanthamoeba using culturing and molecular
approaches. Briefly, samples were filtered and cultured on non nutrient agar covered with heat killed Escherichia coli. The
presence of Acanthamoeba was confirmed by morphological criteria and genus-specific primer sets. Sequences were analyzed
against all eukaryotic nucleotide sequences archived in the GenBank database. Pathogenicity of the strains were detected using
thermotolerance and osmotolerance assays.
Dr. Maryam Niyyati has completed her Ph.D at the age of 31 years from Tehran University of Medical Sciences and she has published more than 25 papers in reputed journals. She is now assistant professor of Shahid Beheshti University of Medical Sciences in Tehran, Iran.
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