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Expression And Role Of Resistin And PPAR-? (Peroxisome Proliferator-Activated Receptor-?) Receptor In Porcine Ovarian Follicles | 3182
ISSN: 2161-0711

Journal of Community Medicine & Health Education
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Expression and role of resistin and PPAR-? (Peroxisome Proliferator-Activated Receptor-?) receptor in porcine ovarian follicles

International Conference on Occupational Health & Safety Summit

Agnieszka Rak-Mardyla

Accepted Abstracts: J Community Med Health Edu

DOI: 10.4172/2161-0711.S1.003

Adipose tissue is not only as a reservoir of energy-rich molecules but also as height specialized, active endocrine organ directly involved in the control of metabolism and reproductive function through a large number of secreted cytokines and hormones including resistin. Resistin gene expression is detected in adipocytes, and its levels are increased in morbidly obese humans compared with lean control subjects. Transcription factor PPAR-γ, which is specific to adipocytes, has been shown to be important in ovarian function and resistin expression also. Using RT-PCR, immunoblotting and ELISA method we measurement expression and direct in vitro effect of resistin and PPAR- γ receptor on steroid hormone secretion and steroid enzyme expression in porcine ovary. In the pig ovaries collected from prepubertal animals resistin expression was increased during follicular development. Concentration of resistin increased during follicular growth, with the highest level in large follicles, and it was correlated with estradiol levels in follicular fluid. Additionally, resistin can modulate ovarian cells steroidogenesis by effect on androgen production. Expression of PPAR-γ receptor was also detected in ovarian follicles, with maximum in large ovarian follicles collected from normal estrus cycle animals. Resistin is new adipokine which was expressed in ovary and control female reproduction. However, the mechanism of resistin action in the ovary will not be able to fully understand until its receptor will not be identified. Supported by the Polish Committee for Scientific Research as a project Iuventus Plus Programme 0343/PO1/2010 and part of 0447/IP1/2011/71 from 2011- 2014 Poland.
Dr. Agnieszka Rak-Mardyła is an Assistant Professor at Department of Physiology and Toxicology of Reproduction, Institute of Zoology, Jagiellonian University in Cracow, Poland.
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