Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar
GET THE APP
Diagnosis Of Recent And Late Toxoplasma Gondii Strain Rh By Two-dimensional Immunoblot Of Chicken Immunoglobulin G And M Profiles | 9554
Journal of Marine Science: Research & Development
Like us on:
Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
is an intracellular apicomplexan parasite that infects a wide range of warm -blooded vertebrate hosts.
The protein expression of
was determined by image analysis of the tachyzoites proteome separated by standard-
one and conventional two-dimentional gel polyacrylamide electrophoresis (2D- PAGE). The macro- and micro- scale gels
of the tachyzoite proteome were stained with Coomassie brilliant blue and the high sensitive Silver stain. Pooled gels were
prepared from tachyzoites of
. A representative gel spanning a pH range of 3-10 of the tachyzoite proteome consisted
of 1306 distinct polypeptide spots. Two-dimensional electrophoresis (2-DE) combined with 2-DE immunoblotting was used
to resolve and compare immunoglobulins (Igs) M & G patterns against Toxoplasma gondii strain RH (mouse virulent strain).
The subsequent transfer of the resolved polypeptide spots of the tachyzoite proteome to immobilon
-P transfer membrane has
been done using electrophoretic blotting. The broader picture of immunologically reactive polypeptide spots of
been revealed by IgM and IgG
antibodies from SPF chickens that were subcutaneously infected with 5x10
strain RH at different times. Total tachyzoite proteins of
were separated by two-dimensional
gel electrophoresis and analyzed by Western blotting for their reactivity with the 7 and 56 days post-infection (dpi) SPF chicken
antisera. Relying upon immunoglobulin M and G, different antigenic determinant patterns were detected. Of the total number of
polypeptide spots analyzed (1306 differentially expressed protein spots), 6.97% were detected and identified as shared antigenic
polypeptide spots on immunoblot profile using IgG and IgM antibodies regardless the time after infection. Furthermore, some
of the immunoreactive polypeptide spots seemed to be related to the stage of infection. Interestingly, we reported the presence of
natural antibodies to toxoplasmic antigens, in addition to the highly common conserved antigenic determinants that reacted with
non-specific secondary antibody; goat anti-chicken IgG (H+L chain specific) antibodies conjugated with horseradish peroxidase.
In conclusion, unique reactive polypeptide spots are promising candidates for designation of molecular markers to discriminate
recent and late chicken infection.
Saeed Elashram is a professor of parasitology at Faculty of Science Kafr El-Sheikh University. He has published so many papers in reputed journals.
He had four registered patents and contributed in many international conferences.
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals