Evolution Of Molecular Methods For Microbial Detection In Seafood | 9499
Journal of Marine Science: Research & Development
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The introduction of molecular techniques in biotechnology has supplied new approaches that could be applied for a quick and
specific detection of pathogens. Since, one of the major concerns in seafood is the presence of pathogenic microorganisms,
the absence of bacteria such as
Salmonella, Vibrio cholerae, V. parahaemolyticus
has to be granted
in sea food. High quality and safety levels are required by the market to ensure that no outbreaks can affect humans health
by consumption of seafood. Testing of raw material and/or final products for the presence of these pathogens using classical
plate methods is time consuming and laborious. Rapid DNA based methods developed last few decades facilitate specific
detection of pathogens. Techniques based on nucleic acid amplification such as PCR and qPCR showed very high sensitivity and
specificity. Other techniques based on DNA hybridization can use specific labelled DNA probes, short stretches of nucleotides
complementary to the target sequences, to detect quickly pathogens. In recent years, biosensors (biological components that bind
or react with a target molecule and transduce this into a decetable signal) played an important role for food analysis and safety
improving seafood quality. Electrochemical biosensors, optical biosensors, and acoustic biosensors have been used, due to the
high sensitivity and specificity obtained. Biosensors are attractive because they can be easily used by non-specialist personnel and
they allow accurate determination. they show the advantage to require tiny volumes, and to shorten the detection time which is
a critical parameter for food industry in preventing food-related illnesses.
Marisa graduated in Natural Sciences at the University of Padua in 1983. Researcher at the University of Udine, Department of Food Sciences
from 1990 to 2005, and Associate Professor of Molecular Biology Techniques from 2005 ongoing. Member of various Academic Comittee and
Commissions. Author of a patent for the detection of
in organic fluids by PCR (1996, C12Q). Coauthor/author of 111 papers;
8 book chapters; 62 papers/abstracts in proceedings, and 46 posters. Chairman and invited speaker at International workshops and conferences.
Research fields: Development of DNA probes for pathogen detection using molecular methods methods (PCR, RT-PCR, DNA array) and biosensors.
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