Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar
Genomic Characterization Of Buffalo Poxvirus B5R Gene, Pakistan | 12951
ISSN: 2161-1165

Epidemiology: Open Access
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Google Scholar citation report
Citations : 1956

Epidemiology: Open Access received 1956 citations as per Google Scholar report

Epidemiology: Open Access peer review process verified at publons
Indexed In
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Genamics JournalSeek
  • SafetyLit
  • Access to Global Online Research in Agriculture (AGORA)
  • Centre for Agriculture and Biosciences International (CABI)
  • RefSeek
  • Hamdard University
  • OCLC- WorldCat
  • CABI full text
  • Cab direct
  • Publons
  • Geneva Foundation for Medical Education and Research
  • Euro Pub
Share This Page

Genomic characterization of Buffalo poxvirus B5R gene, Pakistan

International Conference on Epidemiology and Evolutionary Genetics

Mohsan Ullah

Accepted Abstracts: Epidemiol

DOI: 10.4172/2161-1165.S1.004

B uffalo poxvirus, a strain of vaccinia virus and causative agent of buffalo pox,is a member of the genus Orthopoxvirus family Poxviridae . Infection by buffalo poxvirus is characterized by the lesions on the udder and teats, which may lead to development of mastitis in dairy buffalo and can severely effect the milk production.TheB5R gene of buffalo poxvirus is an integral component of viral replication and an antagonistic to host immune responses as has been demonstrated in vaccinia virus.To ascertain the genetic nature of circulating buffalo poxviruses in Pakistani livestock population, pastules from udder and teats of infected buffalo were collected. The BHK21 cells were infected with the clinical material and 50 blind passages were given, however, cytopathic effects were evident at 15 th passage. The cell supernatants from every fifth passage were stored on FTA QIACard and were shipped to Uppsala Sweden for genetic analysis. The B5R gene was amplified by specific primers and analyzed at both nucleotide and amino acid levels. Phylogenetic analysis indicated that Pakistani strains of buffalo poxvirus were clustered with previously characterized human poxvirus from Pakistan and buffalo poxvirus from India. However, over several passages, three consistent mutations were observed (I2K, N64D and K111E).The genetic relatedness of the human and buffalo poxviruses indicated that the zoonotic aspect of these viruses should not be overlooked especially in close human-livestock interface. Further studies are required to underpin the specific function of these substitutions and their role in switching hosts species.
MohsanUllah has completed his M.phil in Microbiology in 2010 from the University of Agriculture Faisalabad, Pakistan and graduated in Veterinary Medicine from the same University. He is currently doing Master in infection biology at Uppsala University, Sweden. He had 1 publication in good reputed journal and doing master thesis at Uppsala University, Sweden.