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Listeriamonocytogenes (Lm)is an intracellular zoonotic food borne pathogen which causes a severe disease called listeriosis in
high risk groups. However, information is limited about the prevalence and sources of Lmin blue crab meat and crab processing
plants in Maryland. The purpose of this study was to address this data gap. For this study, samples were collected from seven
processing plants in Maryland from May through November (2006-2007). A total of 488 raw crabs, 624 cooked crab meat (crab
meat), and 624 environmental samples were analyzed by standard methods.Lm was isolated from 4.5% of raw crabs, 0.2% of crab
meat and 2.1% of environmental samples. Among the environmental sites, the most contaminated were raw crab coolers (6.4%)
and receiving docks (5.1%). Most of the isolates (97%) were resistant to at least one antimicrobial agent and 66% were resistant to
three or more antimicrobials. Eight different serotypes were found among 76 Lm isolates and the most common serotypes were
4b, 1/2b and 1/2a. Automated EcoRI ribotyping differentiated 11 ribotypes among the 106 Lm isolates. The distribution of the
ribotypes in each processing plant had a unique contamination pattern. A total of 92 ApaIand 88 AscI pulsotypes were observed
among 106 Lm isolates and distinct pulsotypes were observed in raw crabs, crab meat and environmental samples. Ribotypes
and serotypes recovered from crab processing plants included subtypes that have been associated with listeriosis cases in other
food outbreaks. These results indicate that raw crabs and associated surfaces are potentially important initial sources of Lm
contamination in blue crab meat and crab processing plants.
Biography
Listeriamonocytogenes (Lm)is an intracellular zoonotic food borne pathogen which causes a severe disease called listeriosis in
high risk groups. However, information is limited about the prevalence and sources of Lmin blue crab meat and crab processing
plants in Maryland. The purpose of this study was to address this data gap. For this study, samples were collected from seven
processing plants in Maryland from May through November (2006-2007). A total of 488 raw crabs, 624 cooked crab meat (crab
meat), and 624 environmental samples were analyzed by standard methods.Lm was isolated from 4.5% of raw crabs, 0.2% of crab
meat and 2.1% of environmental samples. Among the environmental sites, the most contaminated were raw crab coolers (6.4%)
and receiving docks (5.1%). Most of the isolates (97%) were resistant to at least one antimicrobial agent and 66% were resistant to
three or more antimicrobials. Eight different serotypes were found among 76 Lm isolates and the most common serotypes were
4b, 1/2b and 1/2a. Automated EcoRI ribotyping differentiated 11 ribotypes among the 106 Lm isolates. The distribution of the
ribotypes in each processing plant had a unique contamination pattern. A total of 92 ApaIand 88 AscI pulsotypes were observed
among 106 Lm isolates and distinct pulsotypes were observed in raw crabs, crab meat and environmental samples. Ribotypes
and serotypes recovered from crab processing plants included subtypes that have been associated with listeriosis cases in other
food outbreaks. These results indicate that raw crabs and associated surfaces are potentially important initial sources of Lm
contamination in blue crab meat and crab processing plants.
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