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Molecular evidence of Leishmania major in Sergentomyia minuta in Tunisia

3rd International Conference on Epidemiology & Public Health

Jihene Bettaieb, Kaouther Jaouadi, Wissem Ghawar and Afif Ben Salah

Posters-Accepted Abstracts: Epidemiology (Sunnyvale)

DOI: 10.4172/2161-1165.S1.012

Abstract

Introduction: Phlebotomus species are known to be the transmission vectors of Leishmania (L.) in different areas of the world.
However, some researchers have hypothesized that Sergentomyia (S.) genus phlebotomine sand flies are capable of transmitting
Leishmania parasites.
Materials & Methods: Sand flies were collected from south Tunisia in 2013. DNA was extracted from individual sand flies and
parasite DNA was detected by PCR amplification of the ribosomal internal transcribed spacer 1 and DNA sequencing.
Results: L. infantum DNA was identified in one specimen of S. dreyfussi. This is the first report of Leishmania DNA detection
from naturally infected wild-caught S. dreyfussi. Our finding supports the assumption that L. infantum transmission via
Sergentomyia is possible.
Conclusion: Currently, no local data is available on infecting Sergentomyia ssp determining whether Sergentomyia is a potential
vector of Leishmania is crucial to understanding the parasite–vector transmission cycle in different areas of the world.

Biography

Jihene Bettaieb is working in Laboratory of Medical Epidemiology, Pasteur Institute of Tunis, Tunisia.

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