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Primordial germ cells (PGCs) are the precursors of gametes responsible for genetic transmission to the next generation. They
provide an ideal system for cryopreservation and restoration of biodiversity. Recently, considerable attention has been raised
to isolation, cryopreservation and transplantation of PGCs between species. However, the specification and development of PGCs
remain elusive in marine economic fish species, impeding the development of PGCs manipulation technology in those species.
In order to obtain the basic information of PGCs and put forward the manipulation technology, the development of PGCs was
analized in two marine fish species by germ cell specific genes. We cloned
gene in turbot and the red seabream.
By whole mount in situ hybridization, we have illustrated the specification and migration of the PGCs in turbot and the red
seabream. Besides, we also investigated the function of the red seabream
transcriptional regulatory sequences (
using medaka as a model system. We proved that the
was able to label the PGCs in medaka by GFP-Pmvas3?UTR mRNA
microinjection and transgenic fish production under control of
. Furthermore, we isolated and cryopreserved the labeled
PGCs and spermatogonia successfully. The results serve for the germ cell manipulation and provide an ideal alternative method
for preservation of genetic resource of marine fish.
Qinghua Liu, associated professor, Institute of Oceanology, Chinese Academy of Sciences. Her reseach areas are: fish reproductive physiology,
reproduction and development of marine fish species. She has published more than 40 papers in some reputed journals.
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