The Effect Of Spirulina (fresh And Dry) On Some Biological Factors In And Penaeus Semisulcatus Larvae | 9569
Journal of Marine Science: Research & Development
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Spirulina is a filamentous cyanobacteria with many applications in food and drug industries, as a food in human, aquaculture,
vet and poultry industries. Semi and mass culture of Spirulina carries out in different countries. This study was carried out in
five phases in order to produce this microalga in Iran. The first phase, Spirulina pure stock was imported from Indonesia. After
identification of species, it was cultured in laboratory until we took 20 liters of biomass. The semi-mass culture was carried out in
green house. Cell concentration and size of Spirulina were recorded during culture daily and their growth rates were calculated.
After two weeks, when the size of Spirulina was suitable, biomass of Spirulina was harvested then accumulated Spirulina weighted
and dried in 24 hours in laboratory. In order to microbiological study, the samples of Spirulina (dry and fresh) were cultured on
blood agar medium and coliforms were counted. The chemical composition of produced Spirulina was measured by standard
methods. Fatty acid and amino acid profiles were acquired by GC and HPLC instruments, respectively. The amount of chlorophyll
in Spirulina was determined by spectroscopy method. Also astaxanthin pigment as an important carotenoid was measured by
HPLC in Spirulina and
larvae fed on Spirulina. At final phase of this project, larva fed on produced Spirulina
(biomass and dry powder) was compared to Z plus, microencapsulated Spirulina (M.C.F) and
algae as control. This
experiment was carried from zoa to early post larvae stage then survival and growth rate of larvae were recorded. The growth
rate of larvae was evaluated with ANOVA test and survival rate of treatments was assessed by Log Rank (Mantel -Cox) test. Also
during larvae stage, two parameters of water such as nitrate and nitrite were measured in zoa, mysis and post larvae stages.
Mansoreh Ghaeni has completed his Ph.D. at the age of 27 years from Islamic Azad University Science and Research of Tehran Branch and she is
expert in phycology and aquaculture. Now she is the head of Fisheries department in Islamic Azad University Ahvaz Branch. She has published more
than 50 papers in reputed journals or international conference and has been serving as an editorial board member of marine biology and wetland
journal. She carried out about 10 projects about microalgae culture and aquaculture.
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