alexa
Reach Us +44-1522-440391
Application Of An Immobilization Technique For The Production Of Protease From Bacillus Subtilis | 4601
ISSN: 2157-7625

Journal of Ecosystem & Ecography
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Application of an immobilization technique for the production of protease from Bacillus subtilis

Biodiversity & Sustainable Energy Development-2012

Harini G Krishna Murthy

Accepted Abstracts: J Ecosyst Ecogr

DOI: 10.4172/2157-7625.S1.010

Abstract
As the demand for proteases increases, it was expected that active strains could be used as catalysts in different industries. Alkaline protease produces rapid lysis of viscous exudates, show good results in the treatment of inflammation foci and edemas, accelerates and potentiates the effect of antibiotics and sulfamide preparations and decomposes viruses and bacterial toxins. In the present work, the strain employed was Bacillus Subtilis. The genus Bacillus was erected by Cohn in 1872 to accommodate a bacterium described by C.G. Erhenbergis 1835 as vibrio subtilis. And it had growth at 37 o C, in a shaker incubator. Cheaper sources of both carbon and nitrogen sources are the key attraction for the commercialization of the production process and thus ability of the microbial agent to grow and produce enzymes using these sources has been arguably a point of interest (Mehta. V & Kanekar. P.P et al). Using batch fermentor was done by the mass production of protease (72 Hrs). The optimal conditions for enzyme production were PH-8.0(3273U/ml), citric acid and mannose as carbon sources and yeast extract as nitrogen source along with salts. The enzyme was immobilized on calcium alginate by cell entrapment. Protease immobilization is important in many applications, such as biosensors, bioorganic synthesis and protein hydrolysis, heterogeneous biocatalysts, selective absorbents, controlled released protein drugs, analytical device and solid phase protein chemistry. The optimal reaction temperature of enzyme was 160C (2967U/ml). The immobilization process significantly improved thermal and storage stability (1hr at room temperature and at 5 o c it will be up to 1-2 months) of the free enzyme. These were 5 o C-70 o C. Centrifugation was done at 27 o C, and productivity was improved after immobilization than free enzyme. Immobilized Enzyme activity was 1000% more than free enzyme Activity. The Protein estimated in enzyme assay at 300nM was 47.5KDA.
Biography
Relevant Topics
Leave Your Message 24x7
Top