Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
In the co-evolution, beneficial intestinal and fecal microorganisms (Fecal Microbiota) play an important role in digestion and
absorption of food, immunity, resistance to pathogens, and health maintaining of their hosts. In order to provide new sources
for discovering new leader compounds of drugs, the diversity and bioactivities of cultivable actinobacteria of animal feces have
been studied. Fecal samples of 7 species of carnivorous, omnivorous and phytophagous animal were collected from Yunnan
Wild Animal Park. The purified cultures of actinobacteria were isolated from these samples using 5 media. The 16S rRNA gene
sequences of 623 selected strains were analyzed, and the phylogenetic analysis was carried out. The isolates were identified at a
species level. The study results showed that actinomycete community of each animal feces was different from each other. Thirteen
genera of actinobacetria were identified from feces of both Giant panda (
) and tiger (
). Total 31 genera of actinobacteria were identified from the 7 species of animal feces. Members of Streptomyces were the first
preponderant microbe, cfu/g of dried samples were up to 10
, and distributed in all 7 species of animals. 39 species of the genus
were identified, and
Streptomyces albus, S. albidoflavus, S. griseus, S. hygroscopicus, S. rutgersensis, S. tendae, and S. violaceoruber
etc., were occurred a high frequency. Members of
were identified in 6 species animals.
Rh. corynebacterioides, Rh. corynebacterioides, Rh. equi, Rh. pyridinivorans
were occurred at high
was identified from 5 species of animal feces.
These results indicated that, first, members of the genus
were the widest distribution
and the largest amount; second, composition of actinobacteria with Chemotype IV to IX and globose and bacilliform shapes,
, were the richest diversity, and occurred a high frequency in most part of tested animal feces. These
are distinct features of cultivable fecal actinomycete community differing from those in soil, plant, and marine environment.
Selective isolation methods for fecal actinomycetes, especially un-known actinomycetes, are very important. A large number
of Gram negative bacteria, fungi and even known actinomycetes in animal feces are a main problem for selective isolation of
un-known actinobacteria. In order to eliminate these troubles, and obtain much more un-known actinobacteria for discovering
novel lead compounds, sampling and isolation methods are key points. Based on many tests in our laboratory, first, it is best to
collect fresh fecal samples from wild animals living in original forest and original habitats (this is not easy); second, the fresh
samples have to dry at 25-28
C for 7 to 10 days; third, the dried samples have to be treated at 80
C for 60 min, and the fecal
suspension should be treated with ultrasound wave for 40? at 150W before isolation; fourth, potassium bichromate 50 mg and 5
mg penicillin, or nystatin 50 mg, nalidixic acid 20 mg and 5 mg penicillin for 1000 ml medium, as inhibitors, have to be added in
the isolation medium for inhibiting fungi and Gram negative bacteria; Fifth, in general, the samples should be diluted to 10
, and the optimum dilution concentration for each animal fecal sample should be tested before and all alone; sixth, YIM
212, YIM 171 and HV medium were better for isolation of fecal actinobacteria.
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals