alexa MiR-155 Modulates Morphine-induced Immunosuppression By Targeting SK3 Channel In Microglia
ISSN: 2155-6105

Journal of Addiction Research & Therapy
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

3rd International Conference and Exhibition on Addiction Research & Therapy
August 04-06, 2014 Hilton-Chicago/Northbrook, Chicago, USA

Xiaoni Zhang, Dong Zheng and Ying Peng
Plenary Session: J Addict Res Ther
DOI: 10.4172/2155-6105.S1.015
Abstract
Background: Opioids, especially morphine, have a diversity of effects on immune system. It is well known that morphine has been proved to have immunosuppressive properties both in vivo and in vitro, and is thought to be involved in the increased incidence of infection in heroin addicts. Evidences have shown that microglia, the main active immune defense cell in the central nervous system, plays an essential role in morphine-induced immunosuppression in central nervous system. In addition, recent studies suggest that microRNAs may play a decisive role in the regulation of gene expression during immune responses in microglia. Although numerous studies have shown that multiple factors were involved in morphine-induced immunosuppression, but the precise cellular and molecular mechanisms are still largely unknown. Objective: This study was to investigate the contribution of a specific microRNA, miR-155, in the modulation of the morphine- induced immunosuppression in microglia. Methods: We detected morphine-induced immunosuppressive effects in both heroin abused patients and morphine-treated mice primary microglia. MiR-155 expression levels were measured in morphine-treated microglia. In vitro experiments were performed to demonstrate how miR-155 influences the expression of cytokines (TNF-α, IL-6 and IL-10) in morphine-treated microglia at both mRNA and extracellular levels. Moreover, interactions between miR-155 and downstream targets were further evaluated by using western blot and luciferase reporter assay. Results: Our results showed that morphine could induce immunosuppression by interfering cytokine expression in both heroin abused patients and morphine-treated mice primary microglia cells. Expression of pro-inflammatory cytokines (TNF-α, IL-6) was decreased, while expression of anti-inflammatory cytokine (IL-10) was increased, respectively, compared to control group. We also found that miR-155 was down-regulated in morphine-treated primary microglia. Moreover, up-regulate miR-155 could reverse morphine-induced cytokines expressions by directly targeting SK3 channel in primary microglia. Conclusions: Our results demonstrated a reversal effect of miR-155 on morphine-induced immunosuppression, and suggested that SK3 channel, the direct target of miR-155, was involved in this process
Biography
Xiaoni Zhang has completed her MD at Zhongshan School of Medicine, Sun Yat-Sen University, 2012. She is currently a second-year graduate student pursuing a Master's degree within the Department of Neurology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University. Her research is focused on the molecular mechanisms of morphine-induced immunosuppressive effects
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]nline.com

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version