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|Lais Simino, Mancini M, Panzarin C, Fontana M F, Milanski M, Torsoni M A, Lgnacio-Souza L M and Torsoni A S|
|State University of Campinas, Brazil|
|Posters & Accepted Abstracts: J Obes Weight Loss Ther|
|Background & Aim: Changes in nutritional status in embryonic development and lactation period, as an excessive caloric intake, may lead to a phenomenon known as metabolic programming. Moreover, recent studies have shown that maternal obesity can have transgenerational effects, affecting not only the F1 generation, but also future generations. These effects, transmitted across generations, can be triggered by epigenetics mechanisms, such as miRNA expression. The miRNA Let-7 is shown to be involved in glucose homeostasis and here, we aimed to evaluate its expression in the liver of F1 offspring from obese mothers and the impacts of its modulation to the F2 generation. Methods: Female Swiss mice were fed with a HF or control diet for an adaptation period and through gestation and lactation. Weaned offspring received control diet until d28. Part of female offspring remained in control diet until mating to generate F2 offspring, which were weaned and received control diet until d28. Results: After the adaptation period, F0 females that consumed a HFD were divided in two groups: Obese prone (OP) or obese resistant (OR), according to their weight gain. OP presented higher body weight, adiposity, serum glucose and NEFA than OR. Male and female offspring from OR and OP (OR-O and OP-O) showed an increase in body weight and adiposity at d28, but OP-O presented impaired glucose tolerance and insulin sensitivity, besides higher serum lipid biomarkers. F1 OP-O also had an overexpression in hepatic Let-7 and down-regulation of AMPK, a predicted mRNA target of this miRNA. F2 offspring showed no alteration in body weight and adiposity, but F2 OP-O presented higher fasting glucose as early as d0 and d28, and an elevated liver fat content. Conclusion: Nutritional overload in critical periods of development leads offspring to epigenetic changes that may have transgenerational negative impacts.|
Laís Simino is a Nutritionist and pursuing her PhD at State University of Campinas – UNICAMP. She belongs to the Obesity and Comorbidities Research Center (OCRC) and Laboratory of Metabolic Diseases, a laboratory that has been specializing in fetal programming research, especially triggered by maternal consumption of high fat diets.
Email: [email protected]
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