Bioactivity of Pure Cultures of Bacteria and Yeast in the Background Action of the Water Extract of a Plant Galega orientalis (Lam)Darmohray L M1*, Gonchar M V2
- *Corresponding Author:
- Darmohray L M, Lviv National University of Veterinary Medicine and Biotechnologies named after S.Z. Gzhytskyj, Ukraine
E-mail: [email protected]
Received: 25 May 2015 Accepted: 13 August 2015 Published: 17 August 2015
It was first conducted testing on antimicrobial activity of a plant Galega orientalis (Lam) on the growth pure cultural of bacteria gram positive (Micrococcus luteus), gram negative (Escherichia coli XL1, DH5) and yeasts (saccharomyces cerevisiae W303). The material for the study was dried vegetative mass Galega orientalis (Lam) in the phase of budding and early flowering. In experiment used medium “Endo” for gram negative, medium LB for gram positive and gram negative, and suslo agar for yeasts.
As a result of the experiment was revealed that 20% concentration of aqueous extract of this plant had inhibitory effects on the growth of pure cultures of bacteria and yeast. Antimicrobial effect of this drug on the growth of gram-negative bacteria (Escherichia coli XL1, DH5) were within 20, 0-30,0% (P<0.001), gram-positive bacteria (M. Luteus)-12,0% (P<0.05) and yeast (S. cerevisiae W303)-30,5% (P<0.01) compared with control. It should be noted that in all cases the addition of the drug Galega orientalis (Lam) did not alter the morphology of colonies (colony size) test strains .
The questions of search the new antimicrobial agents, include natural origin, is very actual during last years. Increasing microbiological pure of eating products, feed grinders, veterinarian preparations are one of the urgent task of Scientifics and industrials.
It has proved the influence of different concentrations of aqueous extract of this culture on the growth of pure cultures of gram-positive and gram-negative bacteria and yeast. Install antimicrobial influence 20-30% concentrations of preparation on the bacteria’s growth (Escherichia coli XL1, DH5). Bacterial action of preparation on the bacteria’s (Micrococcus luteus) and yeasts (saccharomyces cerevisiae W303) were lower. It was described the prospects for further investigation of this problem. It has proved possible relationship between the antimicrobial activity of the extract of this plant and bloating of the rumen in ruminants .
To test this hypothesis we need to conduct further comprehensive studies. It is also advisable to extend the range of tested cultures, ways of extractions and the range of concentrations of the studied preparations.