Conservative Efforts through Embryo Culture of a Vulnerable Plant Hemidesmusindicus L.
Indian medicinal plant is having high medicinal importance and is widely collected from emblica Linn and Hemidesmusindicus, species belonging to family Euphorbiaceae endemic to Peninsular India and vulnerable globally. Poor and inconsistent germination of seeds, excessive fruit collection, unsustainable collection of fuel wood, and habitat loss leads to the low regeneration of this species. With an objective to establish the species population in the natural and allied habitats, our present investigation is carried on the conservation of this plant. Since conventional propagation methods were not effective, in vitro propagation of this plant was attempted for the first time. Our present study involves the effective procedures for in vitro conservation of Phyllanthusindo fischeri through embryo culture. Embryos from the mature seeds were used as explants. Although germination was achieved on MS medium containing various growth regulators, maximum response was observed on MS medium fortified with BAP (2.0)+IBA (2.5)mg/l, BAP 2mg/l followed by GAÃ¢ÂÂ 0.1 mg/l and observed for healthy plantlets formation.