IDENTIFICATION OF ELITE RICE GERMPLASM LINES FOR GRAIN PROTEIN CONTENT, SSR BASED GENOTYPING AND DNA FINGERPRINTING
|A. H. Patil1, V. Premi1, V. Sahu1, M. Dubey1, G. R. Sahu2 and G. Chandel1*
|Corresponding Author: G. Chandel, Tel: 91-771-2442069 (o), 09425285039 (m), Fax: 91-771-2442303E-mail: [email protected] , [email protected]|
|Received: 17 January 2014 Accepted: 03 March 2014|
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The present investigation was aimed at characterizing the rice germplasm lines of Chhattisgarh core collection for grain protein content and their morphological and molecular analysis. The protein content analysis resulted in identification of five high protein germplasm lines namely, CGR-436 (11.2%), GP-145-48 (10.68%), CGR-446 (10.43%), CGR-52 (10.35%) and CGR-77 (9.92%). Morphological analysis for the same revealed a wide range of diversity for eight quantitative traits. Further, the genetic diversity was assessed among 58 rice germplasm lines and varieties using 69 alleles generated by 25 SSR primers. DNA fingerprinting of identified high grain protein containing rice lines (≥ 9.0% grain protein) was carried out using 25 polymorphic SSR markers among which SSR marker RM 489 was found to be highly discriminating marker. Graphical genotyping analysis revealed that these markers differed significantly for the genomic constitution contributed by marker alleles A and B. For SSR marker RM215, the genomic constitution was contributed majorly by allele A (99.6%) with little contribution of allele B (0.4%) whereas these alleles contributed almost equally in case of RM447. Marker trait associations by ANOVA single marker analysis revealed six markers to be significantly associated with grain protein content. The high grain protein germplasm lines and associated DNA markers indentified in this study can be used in the breeding program for the improvement of grain protein content in rice. Also the developed DNA fingerprint of the selected elite rice lines generates the base level data and provides the basis as per requirement of Biodiversity act for registration of varieties and landraces.