IN VITRO DEGRADATION OF ELECTROSPINNING PLLA/HA NANOFIBER SCAFFOLDS COMPARED WITH PURE PLLA
|Fariba Mansourizadeh*1, Vahideh miri2, Farzaneh Shahabi2, Farhad Mansourizadeh3, Elmira Pasban4,Asadollah asadi2
|Corresponding Author: Fariba Mansourizade, Department of Biology, Faculty of Biology, University of Kharazmi, Tehran, Iran, E-mail: [email protected]|
|Received: 04 April 2014 Accepted: 05 May 2014|
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Nano biomaterials play a basic role as scaffolds to prepare three dimensional templates and synthetic extracellular matrix environment for tissue regeneration. In this study Poly-L-lactic acid (PLLA)/hydroxyapatite (HA) nano-composite (PHnC) scaffold and PLLA scaffold were fabricated via electrospinning. The scaffolds were characterized by scanning electron microscope (SEM). In vitro degradation behaviors of three-dimensional tissue engineering porous scaffolds have been systematically investigated up to 40 days in phosphate buffer saline solution at 37 degrees C. In vitro degradation of the scaffold in phosphate buffered saline (PBS) was monitored by measuring weight loss, water uptake and pH change of the buffer during the degradation period. The results showed that, the degradation of the scaffold could be markedly influenced by HA nano-particle. It was also observed that acidification of the environment due to the PLLA degradation is suppressed by HA nanoparticles. The results obtained suggested that the PHnC scaffold could be materials of choice for bone tissue engineering.