alexa THE STANDARDIZATION OF PROTOCOL FOR LARGE SCALE PRODUCTION OF SUGARCANE (CO-86032) THROUGH MICRO PROPAGATION


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Research Article

THE STANDARDIZATION OF PROTOCOL FOR LARGE SCALE PRODUCTION OF SUGARCANE (CO-86032) THROUGH MICRO PROPAGATION

Jai Godheja1*, Sudhir K. Shekhar1, D.R. Modi2
  1. Research Scholar, Department of Biotechnology, Babasaheb Bhimrao Ambedkar University, Lucknow
  2. Associate Professor, Department of Biotechnology, Babasaheb Bhimrao Ambedkar University, Lucknow
Corresponding Author: Jai Godheja, Research Scholar, Department of Biotechnology, Babasaheb Bhimrao Ambedkar University, Lucknow, E-mail: [email protected]
Received: 04 August 2014 Accepted: 28 August 2014
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Abstract

Sugarcane belongs to the grass family (Poaceae), an economically important seed plant family that includes maize, wheat, rice, and sorghum and many forage crops. The main product of sugarcane is sucrose, which accumulates in the stalk internodes. India is the second largest producer of sugar in the world. Sugarcane is an important agro-industrial crop in India, occupying 4.0 million hectare area. The average cane yield in India is about 70.0 tonnes per hectare while the sugar recovery is around 10.0 percent. The present study was carried out to standardize micro-propagation of sugarcane CO-86032 for large scale production of sugarcane planting material. Standardization and optimization of shoot induction and root induction medium were the major objectives of the study. Initiation of explant was found maximum in BAP (1 mg/lit) in MS medium. For shoot proliferation & multiplication SM - II media i.e. MS salts supplemented with 0.3 mg/lit BAP, was found to be the best compared to other media prepared. Though number of multiple shoots were more in SM – III media, but was not appropriate for sub-culturing. Best rooting result obtained in RM – III media i.e. ½ strength MS media supplemented with 3 mg/lit NAA & 3 mg/lit IBA. Maximum survival percentage (97.5 %) was found in SM – II media. From the present study it can be concluded that MS media supplemented with 0.3 mg/lit is best for shoot multiplication and for root induction ½ strength MS media supplemented with 3 mg/lit NAA & 3 mg/lit IBA is best. For commercial production of sugarcane ½ strength MS is found to be more economically viable

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