alexa Assessment Of Bound 3-monochloropropanediol (3-MCPD) And Glycidol Content In Fats And Oils By Gas Chromatography-ion Trap Tandem Mass Spectrometry
ISSN: 2150-3494

Chemical Sciences Journal
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

4th European Chemistry Congress
May 11-13, 2017 Barcelona, Spain

M L Fernandez-de Cordova, L Molina-Garcia and E J Llorent-Martinez
Jean University, Spain
Posters & Accepted Abstracts: Chem Sci J
DOI: 10.4172/2150-3494-C1-009
Abstract
An analytical method for determining bound 3-monochloropropanediol (3-MCPD) and glycidol in fats and oils based on gas chromatography-ion trap mass spectrometry (GC-MS/MS) technique has been developed and applied to the study of concentration levels of these compounds in margarine and olive, palm and sunflower oils. 3-MCPD and glycidol are food contaminants that have been classified as carcinogenic by the International Agency for Research on Cancer (IARC). They have been detected in wide range of food such as bread, coffee, pastries, etc., which are produced at high temperatures, as well as in refined animal and vegetable fats and oils, and in foods containing them. In oils and fats, 3-MCPD originates when triglycerides are hydrolyzed to mono- and diglycerides and they then undergo a substitution reaction with chlorine atoms, with temperatures above 140 °C being required. Glycidol was first detected in palm oil due to its high content of diglycerides and the high temperatures used in its refining. In 2014, the European Union (EU) issued a Recommendation to Member States about the need to assess the concentration levels of these contaminants in certain foods, as well as to develop new analytical methods for their determination in order to protect the health of European consumers. The developed method consists of two parallel tests, A and B, which are carried out using basic catalysis. 3-MCPD-d5 is used as internal standard in order to obtain reproducible results. In assay A, both bound 3-MCPD and glycidol are determined. A transesterification of 3-MCPD and glycidol esters is carried out with sodium methoxide in methanol, whereby both compounds are released. The time of this reaction is strictly controlled in order to avoid the conversion of part of 3-MCPD in glycidol. The reaction is stopped by adding an acidic solution of NaCl, which causes the conversion of free glycidol in 3-MCPD. After a clean-up step with isohexane, free 3-MCPD and the internal standard are extracted with ethyl ether:ethyl acetate (6:4, v/v), derivatized with phenylboronic acid (PBA) and determined by GC-MS/MS. The test B is carried out analogously to test A, but in this case the transesterification reaction is stopped by the addition of an acidic solution of a non-chlorinated salt, NaBr. Under these conditions free glycidol reacts to give a product other than 3-MCPD, which does not interfere in its determination. Therefore, in test B only 3-MCPD is determined. The glycidol ester content is determined from the difference between the 3-MCPD ester content calculated in tests A and B. The quantification was performed in MS/MS mode what allowed a significant reduction in the background noise. Ions m/z 91 (147 →91) and m/z 93 (150  93) were selected for the measurement of 3-MCPD phenylboronate and 3-MCPD-d5 phenylboronate, respectively.
Biography

M L Fernández de Córdova is a full professor in Analytical Chemistry at the University of Jaén. She has published more than 75 papers in reputed journals as well as numerous book chapters. Her main researches involve the development of automatic methods of analysis and the determination of contaminants in food.

Email: [email protected]

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords