MiRNCDH- MicroRNA-516-3p, A Novel Circulating MiRNA Differentially Expressed In HCV And HCV Related HCC Egyptian Patients | 109153
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Recommended Conferences

2nd International Conference on Biotechnology and Healthcare

Auckland, New Zealand
Google scholar citation report
Citations : 1536

Journal of Biotechnology & Biomaterials received 1536 citations as per google scholar report

Indexed In
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Open J Gate
  • Genamics JournalSeek
  • Academic Keys
  • ResearchBible
  • China National Knowledge Infrastructure (CNKI)
  • Access to Global Online Research in Agriculture (AGORA)
  • Electronic Journals Library
  • RefSeek
  • Hamdard University
  • OCLC- WorldCat
  • SWB online catalog
  • Virtual Library of Biology (vifabio)
  • Publons
  • Geneva Foundation for Medical Education and Research
  • Euro Pub
Share This Page

miRNCDH- microRNA-516-3p, a novel circulating miRNA differentially expressed in HCV and HCV related HCC Egyptian patients

Samar Samir Youssef, Rana Ahmed Youness, Eman Abd El- Razek Abbas and Mohamed El Kassas

National Research Centre, EgyptGerman University in Cairo, EgyptHelwan University, Egypt

ScientificTracks Abstracts: J Biotechnol Biomater

Liver disorders arising from Hepatitis C virus (HCV) infection are considered a key challenge in the field of translational medicine. HCV and HCV related hepatocellular carcinoma (HCC) poses a heavy burden globally; pivoting on the geographical region, Egypt showed the highest prevalence of HCV worldwide. MicroRNAs (miRNAs) were found to regulate or be regulated by HCV infection. Recently, circulating miRNAs detected in human serum are proposed as novel class of biomarkers for different pathological conditions. miR-516-3p is a novel miRNA that was not widely investigated in terms of infectious diseases rather than oncology. It was recently reported as antimetastamiR through activation of Wnt/B-catenin pathway in gastric cancer. However, among studies efforts has been employed to investigate the alteration in the circulating miRNA pattern that is related with HCV infection and associated HCC, miR-516-3p is scarcely discussed. Moreover, this has not been investigated in case of Egyptian HCV patients or HCC patients. Therefore, the aim of this study is to investigate the expression profile of the novel liverassociated miRNA, miR-516-3p, in the serum of HCV and HCC Egyptian patients. Serum samples were collected from 50 HCV, 20 HCC Egyptian patients and 30 age-matched healthy controls. Total RNA was extracted using Geneaid total RNA mini-kit. Reverse transcription was performed using TaqManŽ MicroRNA Reverse Transcription Kit. Finally, miR-516 expression was quantified using qRT-PCR. Data was statistically analyzed using GraphPad Prism 5.00 software. miR-516-3p was found to be significantly up-regulated in HCV patients compared to agematched healthy controls (p=0.002). Also miR-516-3p showed statistically significant upregulation in HCC versus HCV patients (p=0.0001). This study identifies miR-516-3p as a novel circulating MicroRNA. miR-516-3p was found to be overexpressed in the serum of HCV patients. Moreover miR-516-3p showed escalated up regulation in HCC patients. Thus shedding the light on the pivotal role of miR-516-3p in HCV and HCV induced HCC.

Samar Samir Youssef has been working in National Research Centre, Egypt since 1991. She has completed her PhD at Ain Shams University and Postdoctoral studies in France. She has published more than 25 papers in reputed journals and has been serving as a Reviewer Board Member of reputed journals.

E-mail: [email protected]