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A large variety of conditions can involve the retina and vitreous which are not readily visible, such as uveitis, retinal vasculopathy, retinal detachments or tears, macular holes, macular degeneration, diabetic retinopathy, retinopathy of prematurity, retinitis pigmentosa, retinoblastoma, flashes and floaters, vitreous hemorrhage, and eye tumor. These diseases affect millions of people and endanger their visual function, sometimes irreversibly. A prompt and correct diagnosis of retina and vitreous diseases is critical for restoring the lost vision or maintain the present visual acuity. However, a number of them are still difficult to differentiate with the development of highly technical equipment and testing. For the same patient, clinicians may even have different treatment plans. An overview of various retina and vitreous diseases will provide a comprehensive picture of up-to-date diagnostic and therapeutic strategy. We specifically invite you as an expert in the “Overview of retina and vitreous diseases” to submit an original research, comment, review and opinion article which may contribute to a better understanding in the retina and vitreous diseases and a new direction for the diagnosis and treatment.